{"id":865,"date":"2026-10-12T16:59:39","date_gmt":"2026-10-12T16:59:39","guid":{"rendered":"https:\/\/avo6.com\/?page_id=865"},"modified":"2026-10-12T16:59:40","modified_gmt":"2026-10-12T16:59:40","slug":"biologie-moleculaire","status":"publish","type":"page","link":"https:\/\/avo6.com\/?page_id=865","title":{"rendered":"Biologie mol\u00e9culaire"},"content":{"rendered":"\n<p>Calculs de paillasse en biologie mol\u00e9culaire. Chaque outil affiche ses hypoth\u00e8ses ; le m\u00e9lange PCR calcule les volumes \u00e0 partir des concentrations de votre propre protocole valid\u00e9.<\/p>\n\n<div class=\"a6t-group a6t-has-tabs\" data-a6t-group=\"moleculaire\"><nav class=\"a6t-tabs\" aria-label=\"Outils de cette page\"><ul><li><a href=\"#adn-arn-a260\" data-a6t-tab=\"adn-arn-a260\">ADN\/ARN : A260 et puret\u00e9<\/a><\/li><li><a href=\"#dilution-extrait\" data-a6t-tab=\"dilution-extrait\">Dilution d\u2019extrait<\/a><\/li><li><a href=\"#copies-adn\" data-a6t-tab=\"copies-adn\">Copies d\u2019ADN (masse \u2192 copies)<\/a><\/li><li><a href=\"#eluat-echantillon\" data-a6t-tab=\"eluat-echantillon\">\u00c9luat \u2192 \u00e9chantillon (copies\/mL)<\/a><\/li><li><a href=\"#melange-pcr\" data-a6t-tab=\"melange-pcr\">M\u00e9lange PCR<\/a><\/li><\/ul><\/nav>\t<section class=\"a6t-tool\" id=\"adn-arn-a260\" data-a6t-panel aria-labelledby=\"a6t-adn-arn-a260-title\">\n\t\t<h2 id=\"a6t-adn-arn-a260-title\" class=\"a6t-title\">Dosage d\u2019ADN ou d\u2019ARN par absorbance (A260)<\/h2>\n\t\t<p class=\"a6t-lead\">Concentration (ng\/\u00b5L = \u00b5g\/mL), quantit\u00e9 totale et rapports de puret\u00e9 A260\/A280 et A260\/A230.<\/p>\n\n\t\t<form class=\"calc a6t-form\" data-a6t-tool=\"adn-arn-a260\" novalidate autocomplete=\"off\" aria-label=\"Calculateur : ADN\/ARN : A260 et puret\u00e9\">\n\t\t\t\t\t\t<fieldset class=\"a6t-fieldset\" disabled>\n\t\t\t\t<legend class=\"screen-reader-text\">Donn\u00e9es \u00e0 saisir<\/legend>\n\t\t\t\t<div class=\"field a6t-field\"><label for=\"a6t-adn-arn-a260-type\">Acide nucl\u00e9ique<\/label><select id=\"a6t-adn-arn-a260-type\" name=\"type\" aria-describedby=\"a6t-adn-arn-a260-type-err\"><option value=\"dsdna\" selected='selected'>ADN double brin (50 ng\/\u00b5L par unit\u00e9)<\/option><option value=\"rna\">ARN (40 ng\/\u00b5L par unit\u00e9)<\/option><option value=\"ssdna\">ADN simple brin (33 ng\/\u00b5L par unit\u00e9)<\/option><\/select><span class=\"error\" id=\"a6t-adn-arn-a260-type-err\" data-a6t-err-for=\"type\" aria-live=\"polite\"><\/span><\/div><div class=\"field a6t-field\"><label for=\"a6t-adn-arn-a260-a260\">Absorbance A260 (trajet 1 cm)<\/label><input id=\"a6t-adn-arn-a260-a260\" name=\"a260\" type=\"text\" inputmode=\"decimal\" autocomplete=\"off\" spellcheck=\"false\" value=\"\" aria-describedby=\"a6t-adn-arn-a260-a260-err\" aria-required=\"true\"><span class=\"error\" id=\"a6t-adn-arn-a260-a260-err\" data-a6t-err-for=\"a260\" aria-live=\"polite\"><\/span><\/div><div class=\"field a6t-field\"><label for=\"a6t-adn-arn-a260-dilution\">Facteur de dilution \u2014 facultatif<\/label><input id=\"a6t-adn-arn-a260-dilution\" name=\"dilution\" type=\"text\" inputmode=\"decimal\" autocomplete=\"off\" spellcheck=\"false\" value=\"\" aria-describedby=\"a6t-adn-arn-a260-dilution-hint a6t-adn-arn-a260-dilution-err\"><span class=\"hint\" id=\"a6t-adn-arn-a260-dilution-hint\">1 si mesur\u00e9 pur.<\/span><span class=\"error\" id=\"a6t-adn-arn-a260-dilution-err\" data-a6t-err-for=\"dilution\" aria-live=\"polite\"><\/span><\/div><div class=\"field a6t-field\"><label for=\"a6t-adn-arn-a260-a280\">A280 \u2014 facultatif<\/label><input id=\"a6t-adn-arn-a260-a280\" name=\"a280\" type=\"text\" inputmode=\"decimal\" autocomplete=\"off\" spellcheck=\"false\" value=\"\" aria-describedby=\"a6t-adn-arn-a260-a280-err\"><span class=\"error\" id=\"a6t-adn-arn-a260-a280-err\" data-a6t-err-for=\"a280\" aria-live=\"polite\"><\/span><\/div><div class=\"field a6t-field\"><label for=\"a6t-adn-arn-a260-a230\">A230 \u2014 facultatif<\/label><input id=\"a6t-adn-arn-a260-a230\" name=\"a230\" type=\"text\" inputmode=\"decimal\" autocomplete=\"off\" spellcheck=\"false\" value=\"\" aria-describedby=\"a6t-adn-arn-a260-a230-err\"><span class=\"error\" id=\"a6t-adn-arn-a260-a230-err\" data-a6t-err-for=\"a230\" aria-live=\"polite\"><\/span><\/div><div class=\"field a6t-field\"><label for=\"a6t-adn-arn-a260-vol\">Volume de l\u2019extrait (\u00b5L) \u2014 facultatif<\/label><input id=\"a6t-adn-arn-a260-vol\" name=\"vol\" type=\"text\" inputmode=\"decimal\" autocomplete=\"off\" spellcheck=\"false\" value=\"\" aria-describedby=\"a6t-adn-arn-a260-vol-err\"><span class=\"error\" id=\"a6t-adn-arn-a260-vol-err\" data-a6t-err-for=\"vol\" aria-live=\"polite\"><\/span><\/div>\t\t\t\t<div class=\"a6t-actions\">\n\t\t\t\t\t<button type=\"submit\" class=\"btn btn-primary\">Calculer<\/button>\n\t\t\t\t\t<button type=\"reset\" class=\"btn btn-ghost\">R\u00e9initialiser<\/button>\n\t\t\t\t\t<button type=\"button\" class=\"a6t-linkbtn\" data-a6t-example>Essayer l\u2019exemple<\/button>\n\t\t\t\t<\/div>\n\t\t\t<\/fieldset>\n\t\t\t<p class=\"error a6t-formerror\" data-a6t-err-for=\"_\" role=\"alert\"><\/p>\n\n\t\t\t<div class=\"calc-result a6t-result\" role=\"status\" aria-live=\"polite\" tabindex=\"-1\" hidden>\n\t\t\t\t<div class=\"a6t-main\"><\/div>\n\t\t\t\t<ul class=\"a6t-lines\"><\/ul>\n\t\t\t\t<div class=\"a6t-chart\"><\/div>\n\t\t\t\t<div class=\"a6t-table\"><\/div>\n\t\t\t\t<p class=\"a6t-formula\"><\/p>\n\t\t\t\t<ul class=\"a6t-notes\"><\/ul>\n\t\t\t\t<p class=\"hint\">R\u00e9sultat indicatif, \u00e0 interpr\u00e9ter par un professionnel de sant\u00e9.<\/p>\n\t\t\t\t<div class=\"a6t-result-actions\">\n\t\t\t\t\t<button type=\"button\" class=\"btn btn-ghost\" data-a6t-copy>Copier le r\u00e9sultat<\/button>\n\t\t\t\t\t<span class=\"a6t-copied\" aria-live=\"polite\"><\/span>\n\t\t\t\t<\/div>\n\t\t\t<\/div>\n\n\t\t\t<noscript><p class=\"notice\">Ce calculateur fonctionne dans votre navigateur et n\u00e9cessite JavaScript. La formule et un exemple sont d\u00e9taill\u00e9s ci-dessous.<\/p><\/noscript>\n\t\t\t<script type=\"application\/json\" class=\"a6t-example-data\">{\"type\":\"dsdna\",\"a260\":\"0,5\",\"dilution\":\"10\",\"vol\":\"50\"}<\/script>\n\t\t<\/form>\n\n\t\t<div class=\"a6t-doc\">\n\t\t\t<h3>Formule<\/h3>\n\t\t\t<div class=\"a6t-formula-box\"><p>Concentration (ng\/\u00b5L) = A260 \u00d7 facteur (50, 40 ou 33) \u00d7 dilution<\/p><p>Quantit\u00e9 (ng) = concentration \u00d7 volume (\u00b5L).<\/p><\/div>\n\n\t\t\t<h3>Unit\u00e9s<\/h3>\n\t\t\t<p>1 ng\/\u00b5L = 1 \u00b5g\/mL. Absorbance pour un trajet optique de 1 cm (les microspectrophotom\u00e8tres la normalisent).<\/p>\n\n\t\t\t<h3>Exemple<\/h3>\n\t\t\t<p>ADN double brin, A260 = 0,5 sur une dilution au 1\/10 : 0,5 \u00d7 50 \u00d7 10 = 250 ng\/\u00b5L ; 50 \u00b5L contiennent 12 500 ng.<\/p>\n\n\t\t\t<h3>Limites<\/h3>\n\t\t\t<ul>\n\t\t\t\t\t\t\t\t\t<li>Rep\u00e8res de puret\u00e9 : A260\/A280 \u2248 1,8\u20131,9 (ADN) et \u2248 2,0\u20132,1 (ARN) ; A260\/A230 \u2248 2,0\u20132,4. Ils d\u00e9pendent du pH et du tampon.<\/li>\n\t\t\t\t\t\t\t\t\t<li>L\u2019absorbance ne distingue pas ADN, ARN et nucl\u00e9otides libres ; une m\u00e9thode fluorim\u00e9trique est plus sp\u00e9cifique.<\/li>\n\t\t\t\t\t\t\t<\/ul>\n\n\t\t\t<h3>R\u00e9f\u00e9rences<\/h3>\n\t\t\t<ol class=\"a6t-refs\">\n\t\t\t\t\t\t\t\t\t<li>\n\t\t\t\t\t\t\t\t\t\t\t\t\t<a href=\"https:\/\/agtc.umd.edu\/files\/TechNote_MVS_Analysis_of_Nucleic_Acid_Concentration_and_Purity.pdf\" rel=\"noopener\" target=\"_blank\">Koetsier G, Cantor E. A Practical Guide to Analyzing Nucleic Acid Concentration and Purity with Microvolume Spectrophotometers. New England Biolabs, note technique.<\/a>\n\t\t\t\t\t\t\t\t\t\t\t<\/li>\n\t\t\t\t\t\t\t<\/ol>\n\t\t\t<p class=\"a6t-verified\">Formule v\u00e9rifi\u00e9e par rapport aux r\u00e9f\u00e9rences ci-dessus le 12 octobre 2026.<\/p>\n\t\t<\/div>\n\t<\/section>\n\t\t<section class=\"a6t-tool\" id=\"dilution-extrait\" data-a6t-panel aria-labelledby=\"a6t-dilution-extrait-title\">\n\t\t<h2 id=\"a6t-dilution-extrait-title\" class=\"a6t-title\">Dilution d\u2019un extrait d\u2019ADN ou d\u2019ARN<\/h2>\n\t\t<p class=\"a6t-lead\">Volume d\u2019extrait et de diluant pour obtenir une concentration cible (ng\/\u00b5L) dans un volume final.<\/p>\n\n\t\t<form class=\"calc a6t-form\" data-a6t-tool=\"dilution-extrait\" novalidate autocomplete=\"off\" aria-label=\"Calculateur : Dilution d\u2019extrait\">\n\t\t\t\t\t\t<fieldset class=\"a6t-fieldset\" disabled>\n\t\t\t\t<legend class=\"screen-reader-text\">Donn\u00e9es \u00e0 saisir<\/legend>\n\t\t\t\t<div class=\"field a6t-field\"><label for=\"a6t-dilution-extrait-stock\">Concentration de l\u2019extrait (ng\/\u00b5L)<\/label><input id=\"a6t-dilution-extrait-stock\" name=\"stock\" type=\"text\" inputmode=\"decimal\" autocomplete=\"off\" spellcheck=\"false\" value=\"\" aria-describedby=\"a6t-dilution-extrait-stock-err\" aria-required=\"true\"><span class=\"error\" id=\"a6t-dilution-extrait-stock-err\" data-a6t-err-for=\"stock\" aria-live=\"polite\"><\/span><\/div><div class=\"field a6t-field\"><label for=\"a6t-dilution-extrait-target\">Concentration souhait\u00e9e (ng\/\u00b5L)<\/label><input id=\"a6t-dilution-extrait-target\" name=\"target\" type=\"text\" inputmode=\"decimal\" autocomplete=\"off\" spellcheck=\"false\" value=\"\" aria-describedby=\"a6t-dilution-extrait-target-err\" aria-required=\"true\"><span class=\"error\" id=\"a6t-dilution-extrait-target-err\" data-a6t-err-for=\"target\" aria-live=\"polite\"><\/span><\/div><div class=\"field a6t-field\"><label for=\"a6t-dilution-extrait-vfinal\">Volume final (\u00b5L)<\/label><input id=\"a6t-dilution-extrait-vfinal\" name=\"vfinal\" type=\"text\" inputmode=\"decimal\" autocomplete=\"off\" spellcheck=\"false\" value=\"\" aria-describedby=\"a6t-dilution-extrait-vfinal-err\" aria-required=\"true\"><span class=\"error\" id=\"a6t-dilution-extrait-vfinal-err\" data-a6t-err-for=\"vfinal\" aria-live=\"polite\"><\/span><\/div>\t\t\t\t<div class=\"a6t-actions\">\n\t\t\t\t\t<button type=\"submit\" class=\"btn btn-primary\">Calculer<\/button>\n\t\t\t\t\t<button type=\"reset\" class=\"btn btn-ghost\">R\u00e9initialiser<\/button>\n\t\t\t\t\t<button type=\"button\" class=\"a6t-linkbtn\" data-a6t-example>Essayer l\u2019exemple<\/button>\n\t\t\t\t<\/div>\n\t\t\t<\/fieldset>\n\t\t\t<p class=\"error a6t-formerror\" data-a6t-err-for=\"_\" role=\"alert\"><\/p>\n\n\t\t\t<div class=\"calc-result a6t-result\" role=\"status\" aria-live=\"polite\" tabindex=\"-1\" hidden>\n\t\t\t\t<div class=\"a6t-main\"><\/div>\n\t\t\t\t<ul class=\"a6t-lines\"><\/ul>\n\t\t\t\t<div class=\"a6t-chart\"><\/div>\n\t\t\t\t<div class=\"a6t-table\"><\/div>\n\t\t\t\t<p class=\"a6t-formula\"><\/p>\n\t\t\t\t<ul class=\"a6t-notes\"><\/ul>\n\t\t\t\t<p class=\"hint\">R\u00e9sultat indicatif, \u00e0 interpr\u00e9ter par un professionnel de sant\u00e9.<\/p>\n\t\t\t\t<div class=\"a6t-result-actions\">\n\t\t\t\t\t<button type=\"button\" class=\"btn btn-ghost\" data-a6t-copy>Copier le r\u00e9sultat<\/button>\n\t\t\t\t\t<span class=\"a6t-copied\" aria-live=\"polite\"><\/span>\n\t\t\t\t<\/div>\n\t\t\t<\/div>\n\n\t\t\t<noscript><p class=\"notice\">Ce calculateur fonctionne dans votre navigateur et n\u00e9cessite JavaScript. La formule et un exemple sont d\u00e9taill\u00e9s ci-dessous.<\/p><\/noscript>\n\t\t\t<script type=\"application\/json\" class=\"a6t-example-data\">{\"stock\":\"250\",\"target\":\"10\",\"vfinal\":\"100\"}<\/script>\n\t\t<\/form>\n\n\t\t<div class=\"a6t-doc\">\n\t\t\t<h3>Formule<\/h3>\n\t\t\t<div class=\"a6t-formula-box\"><p>Volume d\u2019extrait = concentration cible \u00d7 volume final \/ concentration de l\u2019extrait ; diluant = volume final \u2212 volume d\u2019extrait.<\/p><\/div>\n\n\t\t\t<h3>Unit\u00e9s<\/h3>\n\t\t\t<p>ng\/\u00b5L et \u00b5L.<\/p>\n\n\t\t\t<h3>Exemple<\/h3>\n\t\t\t<p>Extrait \u00e0 250 ng\/\u00b5L, cible 10 ng\/\u00b5L dans 100 \u00b5L : pr\u00e9lever 4 \u00b5L et ajouter 96 \u00b5L de diluant.<\/p>\n\n\t\t\t<h3>Limites<\/h3>\n\t\t\t<ul>\n\t\t\t\t\t\t\t\t\t<li>Un volume d\u2019extrait inf\u00e9rieur \u00e0 1 \u00b5L est peu pr\u00e9cis : pr\u00e9voir une dilution interm\u00e9diaire.<\/li>\n\t\t\t\t\t\t\t\t\t<li>Diluer dans le tampon recommand\u00e9 par le protocole (eau sans nucl\u00e9ase, TE\u2026).<\/li>\n\t\t\t\t\t\t\t<\/ul>\n\n\t\t\t<h3>R\u00e9f\u00e9rences<\/h3>\n\t\t\t<ol class=\"a6t-refs\">\n\t\t\t\t\t\t\t\t\t<li>\n\t\t\t\t\t\t\t\t\t\t\t\t\t<a href=\"https:\/\/goldbook.iupac.org\/\" rel=\"noopener\" target=\"_blank\">IUPAC. Compendium of Chemical Terminology (Gold Book) \u2014 concentration en quantit\u00e9 de mati\u00e8re, concentration massique, fraction massique, fraction volumique.<\/a>\n\t\t\t\t\t\t\t\t\t\t\t<\/li>\n\t\t\t\t\t\t\t<\/ol>\n\t\t\t<p class=\"a6t-verified\">Formule v\u00e9rifi\u00e9e par rapport aux r\u00e9f\u00e9rences ci-dessus le 12 octobre 2026.<\/p>\n\t\t<\/div>\n\t<\/section>\n\t\t<section class=\"a6t-tool\" id=\"copies-adn\" data-a6t-panel aria-labelledby=\"a6t-copies-adn-title\">\n\t\t<h2 id=\"a6t-copies-adn-title\" class=\"a6t-title\">Nombre de copies d\u2019ADN double brin \u00e0 partir de la masse<\/h2>\n\t\t<p class=\"a6t-lead\">Nombre de copies d\u2019un fragment ou d\u2019un plasmide d\u2019ADN double brin \u00e0 partir de sa masse et de sa longueur.<\/p>\n\n\t\t<form class=\"calc a6t-form\" data-a6t-tool=\"copies-adn\" novalidate autocomplete=\"off\" aria-label=\"Calculateur : Copies d\u2019ADN (masse \u2192 copies)\">\n\t\t\t\t\t\t<fieldset class=\"a6t-fieldset\" disabled>\n\t\t\t\t<legend class=\"screen-reader-text\">Donn\u00e9es \u00e0 saisir<\/legend>\n\t\t\t\t<div class=\"field a6t-field\"><label for=\"a6t-copies-adn-mass\">Masse d\u2019ADN (ng)<\/label><input id=\"a6t-copies-adn-mass\" name=\"mass\" type=\"text\" inputmode=\"decimal\" autocomplete=\"off\" spellcheck=\"false\" value=\"\" aria-describedby=\"a6t-copies-adn-mass-err\" aria-required=\"true\"><span class=\"error\" id=\"a6t-copies-adn-mass-err\" data-a6t-err-for=\"mass\" aria-live=\"polite\"><\/span><\/div><div class=\"field a6t-field\"><label for=\"a6t-copies-adn-lengthBp\">Longueur (paires de bases)<\/label><input id=\"a6t-copies-adn-lengthBp\" name=\"lengthBp\" type=\"text\" inputmode=\"numeric\" autocomplete=\"off\" spellcheck=\"false\" value=\"\" aria-describedby=\"a6t-copies-adn-lengthBp-err\" aria-required=\"true\"><span class=\"error\" id=\"a6t-copies-adn-lengthBp-err\" data-a6t-err-for=\"lengthBp\" aria-live=\"polite\"><\/span><\/div><div class=\"field a6t-field\"><label for=\"a6t-copies-adn-vol\">Volume (\u00b5L) \u2014 facultatif, pour copies\/\u00b5L<\/label><input id=\"a6t-copies-adn-vol\" name=\"vol\" type=\"text\" inputmode=\"decimal\" autocomplete=\"off\" spellcheck=\"false\" value=\"\" aria-describedby=\"a6t-copies-adn-vol-err\"><span class=\"error\" id=\"a6t-copies-adn-vol-err\" data-a6t-err-for=\"vol\" aria-live=\"polite\"><\/span><\/div>\t\t\t\t<div class=\"a6t-actions\">\n\t\t\t\t\t<button type=\"submit\" class=\"btn btn-primary\">Calculer<\/button>\n\t\t\t\t\t<button type=\"reset\" class=\"btn btn-ghost\">R\u00e9initialiser<\/button>\n\t\t\t\t\t<button type=\"button\" class=\"a6t-linkbtn\" data-a6t-example>Essayer l\u2019exemple<\/button>\n\t\t\t\t<\/div>\n\t\t\t<\/fieldset>\n\t\t\t<p class=\"error a6t-formerror\" data-a6t-err-for=\"_\" role=\"alert\"><\/p>\n\n\t\t\t<div class=\"calc-result a6t-result\" role=\"status\" aria-live=\"polite\" tabindex=\"-1\" hidden>\n\t\t\t\t<div class=\"a6t-main\"><\/div>\n\t\t\t\t<ul class=\"a6t-lines\"><\/ul>\n\t\t\t\t<div class=\"a6t-chart\"><\/div>\n\t\t\t\t<div class=\"a6t-table\"><\/div>\n\t\t\t\t<p class=\"a6t-formula\"><\/p>\n\t\t\t\t<ul class=\"a6t-notes\"><\/ul>\n\t\t\t\t<p class=\"hint\">R\u00e9sultat indicatif, \u00e0 interpr\u00e9ter par un professionnel de sant\u00e9.<\/p>\n\t\t\t\t<div class=\"a6t-result-actions\">\n\t\t\t\t\t<button type=\"button\" class=\"btn btn-ghost\" data-a6t-copy>Copier le r\u00e9sultat<\/button>\n\t\t\t\t\t<span class=\"a6t-copied\" aria-live=\"polite\"><\/span>\n\t\t\t\t<\/div>\n\t\t\t<\/div>\n\n\t\t\t<noscript><p class=\"notice\">Ce calculateur fonctionne dans votre navigateur et n\u00e9cessite JavaScript. La formule et un exemple sont d\u00e9taill\u00e9s ci-dessous.<\/p><\/noscript>\n\t\t\t<script type=\"application\/json\" class=\"a6t-example-data\">{\"mass\":\"1\",\"lengthBp\":\"3000\"}<\/script>\n\t\t<\/form>\n\n\t\t<div class=\"a6t-doc\">\n\t\t\t<h3>Formule<\/h3>\n\t\t\t<div class=\"a6t-formula-box\"><p>Masse molaire (g\/mol) = pb \u00d7 617,96 + 36,04<\/p><p>Copies = masse (g) \/ masse molaire \u00d7 6,022 \u00d7 10\u00b2\u00b3<\/p><\/div>\n\n\t\t\t<h3>Unit\u00e9s<\/h3>\n\t\t\t<p>Masse en ng ; longueur en paires de bases.<\/p>\n\n\t\t\t<h3>Exemple<\/h3>\n\t\t\t<p>1 ng d\u2019un fragment de 3 000 pb : 10\u207b\u2079 \/ (3 000 \u00d7 617,96 + 36,04) \u00d7 6,022 \u00d7 10\u00b2\u00b3 = 3,25 \u00d7 10\u2078 copies.<\/p>\n\n\t\t\t<h3>Limites<\/h3>\n\t\t\t<ul>\n\t\t\t\t\t\t\t\t\t<li>Hypoth\u00e8ses : ADN double brin, composition moyenne en bases, masse mesur\u00e9e exacte. Ne s\u2019applique pas tel quel \u00e0 l\u2019ARN ni \u00e0 l\u2019ADN simple brin.<\/li>\n\t\t\t\t\t\t\t<\/ul>\n\n\t\t\t<h3>R\u00e9f\u00e9rences<\/h3>\n\t\t\t<ol class=\"a6t-refs\">\n\t\t\t\t\t\t\t\t\t<li>\n\t\t\t\t\t\t\t\t\t\t\t\t\t<a href=\"https:\/\/www.qiagen.com\/en\/applications\/enzymes\/tools-and-calculators\/dsdna-mass-to-moles-conventer\" rel=\"noopener\" target=\"_blank\">QIAGEN. dsDNA Mass \u2194 Moles Converter (masse molaire = pb \u00d7 617,96 + 36,04 g\/mol).<\/a>\n\t\t\t\t\t\t\t\t\t\t\t<\/li>\n\t\t\t\t\t\t\t<\/ol>\n\t\t\t<p class=\"a6t-verified\">Formule v\u00e9rifi\u00e9e par rapport aux r\u00e9f\u00e9rences ci-dessus le 12 octobre 2026.<\/p>\n\t\t<\/div>\n\t<\/section>\n\t\t<section class=\"a6t-tool\" id=\"eluat-echantillon\" data-a6t-panel aria-labelledby=\"a6t-eluat-echantillon-title\">\n\t\t<h2 id=\"a6t-eluat-echantillon-title\" class=\"a6t-title\">De l\u2019\u00e9luat \u00e0 l\u2019\u00e9chantillon : copies\/mL<\/h2>\n\t\t<p class=\"a6t-lead\">Copies\/mL dans l\u2019\u00e9chantillon de d\u00e9part \u00e0 partir des copies par r\u00e9action, des volumes de matrice, d\u2019\u00e9lution et d\u2019\u00e9chantillon extrait.<\/p>\n\n\t\t<form class=\"calc a6t-form\" data-a6t-tool=\"eluat-echantillon\" novalidate autocomplete=\"off\" aria-label=\"Calculateur : \u00c9luat \u2192 \u00e9chantillon (copies\/mL)\">\n\t\t\t\t\t\t<fieldset class=\"a6t-fieldset\" disabled>\n\t\t\t\t<legend class=\"screen-reader-text\">Donn\u00e9es \u00e0 saisir<\/legend>\n\t\t\t\t<div class=\"field a6t-field\"><label for=\"a6t-eluat-echantillon-copiesRxn\">Copies mesur\u00e9es par r\u00e9action<\/label><input id=\"a6t-eluat-echantillon-copiesRxn\" name=\"copiesRxn\" type=\"text\" inputmode=\"decimal\" autocomplete=\"off\" spellcheck=\"false\" value=\"\" aria-describedby=\"a6t-eluat-echantillon-copiesRxn-err\" aria-required=\"true\"><span class=\"error\" id=\"a6t-eluat-echantillon-copiesRxn-err\" data-a6t-err-for=\"copiesRxn\" aria-live=\"polite\"><\/span><\/div><div class=\"field a6t-field\"><label for=\"a6t-eluat-echantillon-vTemplate\">Volume d\u2019\u00e9luat mis dans la r\u00e9action (\u00b5L)<\/label><input id=\"a6t-eluat-echantillon-vTemplate\" name=\"vTemplate\" type=\"text\" inputmode=\"decimal\" autocomplete=\"off\" spellcheck=\"false\" value=\"\" aria-describedby=\"a6t-eluat-echantillon-vTemplate-err\" aria-required=\"true\"><span class=\"error\" id=\"a6t-eluat-echantillon-vTemplate-err\" data-a6t-err-for=\"vTemplate\" aria-live=\"polite\"><\/span><\/div><div class=\"field a6t-field\"><label for=\"a6t-eluat-echantillon-vElution\">Volume d\u2019\u00e9lution (\u00b5L)<\/label><input id=\"a6t-eluat-echantillon-vElution\" name=\"vElution\" type=\"text\" inputmode=\"decimal\" autocomplete=\"off\" spellcheck=\"false\" value=\"\" aria-describedby=\"a6t-eluat-echantillon-vElution-err\" aria-required=\"true\"><span class=\"error\" id=\"a6t-eluat-echantillon-vElution-err\" data-a6t-err-for=\"vElution\" aria-live=\"polite\"><\/span><\/div><div class=\"field a6t-field\"><label for=\"a6t-eluat-echantillon-vSample\">Volume d\u2019\u00e9chantillon extrait<\/label><div class=\"input-unit\"><input id=\"a6t-eluat-echantillon-vSample\" name=\"vSample\" type=\"text\" inputmode=\"decimal\" autocomplete=\"off\" spellcheck=\"false\" value=\"\" aria-describedby=\"a6t-eluat-echantillon-vSample-err\" aria-required=\"true\"><select id=\"a6t-eluat-echantillon-sampleUnit\" name=\"sampleUnit\" aria-label=\"Unit\u00e9 \u2014 Volume d\u2019\u00e9chantillon extrait\"><option value=\"mL\" selected='selected'>mL<\/option><option value=\"\u00b5L\">\u00b5L<\/option><\/select><\/div><span class=\"error\" id=\"a6t-eluat-echantillon-vSample-err\" data-a6t-err-for=\"vSample sampleUnit\" aria-live=\"polite\"><\/span><\/div>\t\t\t\t<div class=\"a6t-actions\">\n\t\t\t\t\t<button type=\"submit\" class=\"btn btn-primary\">Calculer<\/button>\n\t\t\t\t\t<button type=\"reset\" class=\"btn btn-ghost\">R\u00e9initialiser<\/button>\n\t\t\t\t\t<button type=\"button\" class=\"a6t-linkbtn\" data-a6t-example>Essayer l\u2019exemple<\/button>\n\t\t\t\t<\/div>\n\t\t\t<\/fieldset>\n\t\t\t<p class=\"error a6t-formerror\" data-a6t-err-for=\"_\" role=\"alert\"><\/p>\n\n\t\t\t<div class=\"calc-result a6t-result\" role=\"status\" aria-live=\"polite\" tabindex=\"-1\" hidden>\n\t\t\t\t<div class=\"a6t-main\"><\/div>\n\t\t\t\t<ul class=\"a6t-lines\"><\/ul>\n\t\t\t\t<div class=\"a6t-chart\"><\/div>\n\t\t\t\t<div class=\"a6t-table\"><\/div>\n\t\t\t\t<p class=\"a6t-formula\"><\/p>\n\t\t\t\t<ul class=\"a6t-notes\"><\/ul>\n\t\t\t\t<p class=\"hint\">R\u00e9sultat indicatif, \u00e0 interpr\u00e9ter par un professionnel de sant\u00e9.<\/p>\n\t\t\t\t<div class=\"a6t-result-actions\">\n\t\t\t\t\t<button type=\"button\" class=\"btn btn-ghost\" data-a6t-copy>Copier le r\u00e9sultat<\/button>\n\t\t\t\t\t<span class=\"a6t-copied\" aria-live=\"polite\"><\/span>\n\t\t\t\t<\/div>\n\t\t\t<\/div>\n\n\t\t\t<noscript><p class=\"notice\">Ce calculateur fonctionne dans votre navigateur et n\u00e9cessite JavaScript. La formule et un exemple sont d\u00e9taill\u00e9s ci-dessous.<\/p><\/noscript>\n\t\t\t<script type=\"application\/json\" class=\"a6t-example-data\">{\"copiesRxn\":\"500\",\"vTemplate\":\"5\",\"vElution\":\"60\",\"vSample\":\"0,2\",\"sampleUnit\":\"mL\"}<\/script>\n\t\t<\/form>\n\n\t\t<div class=\"a6t-doc\">\n\t\t\t<h3>Formule<\/h3>\n\t\t\t<div class=\"a6t-formula-box\"><p>Copies\/\u00b5L d\u2019\u00e9luat = copies par r\u00e9action \/ volume d\u2019\u00e9luat dans la r\u00e9action<\/p><p>Copies\/mL d\u2019\u00e9chantillon = copies\/\u00b5L d\u2019\u00e9luat \u00d7 volume d\u2019\u00e9lution (\u00b5L) \/ volume extrait (mL)<\/p><\/div>\n\n\t\t\t<h3>Unit\u00e9s<\/h3>\n\t\t\t<p>Volumes en \u00b5L (\u00e9luat, \u00e9lution) et mL ou \u00b5L (\u00e9chantillon).<\/p>\n\n\t\t\t<h3>Exemple<\/h3>\n\t\t\t<p>500 copies dans 5 \u00b5L d\u2019\u00e9luat, \u00e9lution 60 \u00b5L, 0,2 mL extrait : 100 copies\/\u00b5L \u00d7 60 \/ 0,2 = 3,00 \u00d7 10\u2074 copies\/mL.<\/p>\n\n\t\t\t<h3>Limites<\/h3>\n\t\t\t<ul>\n\t\t\t\t\t\t\t\t\t<li>Hypoth\u00e8ses explicites : rendement d\u2019extraction de 100 %, aucune autre dilution, volumes exacts.<\/li>\n\t\t\t\t\t\t\t\t\t<li>Un laboratoire rend ses r\u00e9sultats selon sa m\u00e9thode valid\u00e9e (\u00e9talonnage, unit\u00e9s internationales) : ce calcul ne s\u2019y substitue pas.<\/li>\n\t\t\t\t\t\t\t<\/ul>\n\n\t\t\t<h3>R\u00e9f\u00e9rences<\/h3>\n\t\t\t<ol class=\"a6t-refs\">\n\t\t\t\t\t\t\t\t\t<li>\n\t\t\t\t\t\t\t\t\t\t\t\t\t<a href=\"https:\/\/doi.org\/10.1373\/clinchem.2008.112797\" rel=\"noopener\" target=\"_blank\">Bustin SA, Benes V, Garson JA, et al. The MIQE guidelines: minimum information for publication of quantitative real-time PCR experiments. Clin Chem. 2009;55(4):611-622.<\/a>\n\t\t\t\t\t\t\t\t\t\t\t<\/li>\n\t\t\t\t\t\t\t<\/ol>\n\t\t\t<p class=\"a6t-verified\">Formule v\u00e9rifi\u00e9e par rapport aux r\u00e9f\u00e9rences ci-dessus le 12 octobre 2026.<\/p>\n\t\t<\/div>\n\t<\/section>\n\t\t<section class=\"a6t-tool\" id=\"melange-pcr\" data-a6t-panel aria-labelledby=\"a6t-melange-pcr-title\">\n\t\t<h2 id=\"a6t-melange-pcr-title\" class=\"a6t-title\">M\u00e9lange r\u00e9actionnel PCR (selon votre protocole)<\/h2>\n\t\t<p class=\"a6t-lead\">Volumes par r\u00e9action et volume total de m\u00e9lange \u00e0 partir des concentrations m\u00e8res et finales de votre protocole.<\/p>\n\n\t\t<form class=\"calc a6t-form\" data-a6t-tool=\"melange-pcr\" novalidate autocomplete=\"off\" aria-label=\"Calculateur : M\u00e9lange PCR\">\n\t\t\t\t\t\t\t<p class=\"hint a6t-intro\">Saisissez les composants et concentrations de votre protocole valid\u00e9. L\u2019outil ne propose aucune composition par d\u00e9faut.<\/p>\n\t\t\t\t\t\t<fieldset class=\"a6t-fieldset\" disabled>\n\t\t\t\t<legend class=\"screen-reader-text\">Donn\u00e9es \u00e0 saisir<\/legend>\n\t\t\t\t<div class=\"field a6t-field\"><label for=\"a6t-melange-pcr-vRxn\">Volume r\u00e9actionnel (\u00b5L)<\/label><input id=\"a6t-melange-pcr-vRxn\" name=\"vRxn\" type=\"text\" inputmode=\"decimal\" autocomplete=\"off\" spellcheck=\"false\" value=\"\" aria-describedby=\"a6t-melange-pcr-vRxn-err\" aria-required=\"true\"><span class=\"error\" id=\"a6t-melange-pcr-vRxn-err\" data-a6t-err-for=\"vRxn\" aria-live=\"polite\"><\/span><\/div><div class=\"field a6t-field\"><label for=\"a6t-melange-pcr-nRxn\">Nombre de r\u00e9actions (t\u00e9moins compris)<\/label><input id=\"a6t-melange-pcr-nRxn\" name=\"nRxn\" type=\"text\" inputmode=\"numeric\" autocomplete=\"off\" spellcheck=\"false\" value=\"\" aria-describedby=\"a6t-melange-pcr-nRxn-err\" aria-required=\"true\"><span class=\"error\" id=\"a6t-melange-pcr-nRxn-err\" data-a6t-err-for=\"nRxn\" aria-live=\"polite\"><\/span><\/div><div class=\"field a6t-field\"><label for=\"a6t-melange-pcr-excess\">Exc\u00e8s (%) \u2014 facultatif<\/label><input id=\"a6t-melange-pcr-excess\" name=\"excess\" type=\"text\" inputmode=\"decimal\" autocomplete=\"off\" spellcheck=\"false\" value=\"\" aria-describedby=\"a6t-melange-pcr-excess-hint a6t-melange-pcr-excess-err\"><span class=\"hint\" id=\"a6t-melange-pcr-excess-hint\">10 % si vide.<\/span><span class=\"error\" id=\"a6t-melange-pcr-excess-err\" data-a6t-err-for=\"excess\" aria-live=\"polite\"><\/span><\/div><div class=\"field a6t-field\"><label for=\"a6t-melange-pcr-vTemplate\">Volume de matrice par r\u00e9action (\u00b5L)<\/label><input id=\"a6t-melange-pcr-vTemplate\" name=\"vTemplate\" type=\"text\" inputmode=\"decimal\" autocomplete=\"off\" spellcheck=\"false\" value=\"\" aria-describedby=\"a6t-melange-pcr-vTemplate-err\" aria-required=\"true\"><span class=\"error\" id=\"a6t-melange-pcr-vTemplate-err\" data-a6t-err-for=\"vTemplate\" aria-live=\"polite\"><\/span><\/div><fieldset class=\"field a6t-field a6t-rows\"><legend>Composants du protocole<\/legend><div class=\"a6t-rows-scroll\"><table><thead><tr><th scope=\"col\"><span class=\"screen-reader-text\">N\u00b0<\/span><\/th><th scope=\"col\">Composant<\/th><th scope=\"col\">Concentration m\u00e8re<\/th><th scope=\"col\">Concentration finale<\/th><th scope=\"col\">Unit\u00e9 (identique)<\/th><\/tr><\/thead><tbody><tr><th scope=\"row\" class=\"a6t-rownum\">1<\/th><td><input type=\"text\" name=\"comp1_name\" aria-label=\"Composant, ligne 1\" autocomplete=\"off\" spellcheck=\"false\" placeholder=\"ex. Tampon\"><\/td><td><input type=\"text\" name=\"comp1_stock\" aria-label=\"Concentration m\u00e8re, ligne 1\" autocomplete=\"off\" spellcheck=\"false\" inputmode=\"decimal\"><\/td><td><input type=\"text\" name=\"comp1_final\" aria-label=\"Concentration finale, ligne 1\" autocomplete=\"off\" spellcheck=\"false\" inputmode=\"decimal\"><\/td><td><input type=\"text\" name=\"comp1_unit\" aria-label=\"Unit\u00e9 (identique), ligne 1\" autocomplete=\"off\" spellcheck=\"false\" placeholder=\"\u00b5M, mM, \u00d7, U\/\u00b5L\"><\/td><\/tr><tr><th scope=\"row\" class=\"a6t-rownum\">2<\/th><td><input type=\"text\" name=\"comp2_name\" aria-label=\"Composant, ligne 2\" autocomplete=\"off\" spellcheck=\"false\"><\/td><td><input type=\"text\" name=\"comp2_stock\" aria-label=\"Concentration m\u00e8re, ligne 2\" autocomplete=\"off\" spellcheck=\"false\" inputmode=\"decimal\"><\/td><td><input type=\"text\" name=\"comp2_final\" aria-label=\"Concentration finale, ligne 2\" autocomplete=\"off\" spellcheck=\"false\" inputmode=\"decimal\"><\/td><td><input type=\"text\" name=\"comp2_unit\" aria-label=\"Unit\u00e9 (identique), ligne 2\" autocomplete=\"off\" spellcheck=\"false\"><\/td><\/tr><tr><th scope=\"row\" class=\"a6t-rownum\">3<\/th><td><input type=\"text\" name=\"comp3_name\" aria-label=\"Composant, ligne 3\" autocomplete=\"off\" spellcheck=\"false\"><\/td><td><input type=\"text\" name=\"comp3_stock\" aria-label=\"Concentration m\u00e8re, ligne 3\" autocomplete=\"off\" spellcheck=\"false\" inputmode=\"decimal\"><\/td><td><input type=\"text\" name=\"comp3_final\" aria-label=\"Concentration finale, ligne 3\" autocomplete=\"off\" spellcheck=\"false\" inputmode=\"decimal\"><\/td><td><input type=\"text\" name=\"comp3_unit\" aria-label=\"Unit\u00e9 (identique), ligne 3\" autocomplete=\"off\" spellcheck=\"false\"><\/td><\/tr><tr><th scope=\"row\" class=\"a6t-rownum\">4<\/th><td><input type=\"text\" name=\"comp4_name\" aria-label=\"Composant, ligne 4\" autocomplete=\"off\" spellcheck=\"false\"><\/td><td><input type=\"text\" name=\"comp4_stock\" aria-label=\"Concentration m\u00e8re, ligne 4\" autocomplete=\"off\" spellcheck=\"false\" inputmode=\"decimal\"><\/td><td><input type=\"text\" name=\"comp4_final\" aria-label=\"Concentration finale, ligne 4\" autocomplete=\"off\" spellcheck=\"false\" inputmode=\"decimal\"><\/td><td><input type=\"text\" name=\"comp4_unit\" aria-label=\"Unit\u00e9 (identique), ligne 4\" autocomplete=\"off\" spellcheck=\"false\"><\/td><\/tr><tr><th scope=\"row\" class=\"a6t-rownum\">5<\/th><td><input type=\"text\" name=\"comp5_name\" aria-label=\"Composant, ligne 5\" autocomplete=\"off\" spellcheck=\"false\"><\/td><td><input type=\"text\" name=\"comp5_stock\" aria-label=\"Concentration m\u00e8re, ligne 5\" autocomplete=\"off\" spellcheck=\"false\" inputmode=\"decimal\"><\/td><td><input type=\"text\" name=\"comp5_final\" aria-label=\"Concentration finale, ligne 5\" autocomplete=\"off\" spellcheck=\"false\" inputmode=\"decimal\"><\/td><td><input type=\"text\" name=\"comp5_unit\" aria-label=\"Unit\u00e9 (identique), ligne 5\" autocomplete=\"off\" spellcheck=\"false\"><\/td><\/tr><tr><th scope=\"row\" class=\"a6t-rownum\">6<\/th><td><input type=\"text\" name=\"comp6_name\" aria-label=\"Composant, ligne 6\" autocomplete=\"off\" spellcheck=\"false\"><\/td><td><input type=\"text\" name=\"comp6_stock\" aria-label=\"Concentration m\u00e8re, ligne 6\" autocomplete=\"off\" spellcheck=\"false\" inputmode=\"decimal\"><\/td><td><input type=\"text\" name=\"comp6_final\" aria-label=\"Concentration finale, ligne 6\" autocomplete=\"off\" spellcheck=\"false\" inputmode=\"decimal\"><\/td><td><input type=\"text\" name=\"comp6_unit\" aria-label=\"Unit\u00e9 (identique), ligne 6\" autocomplete=\"off\" spellcheck=\"false\"><\/td><\/tr><tr><th scope=\"row\" class=\"a6t-rownum\">7<\/th><td><input type=\"text\" name=\"comp7_name\" aria-label=\"Composant, ligne 7\" autocomplete=\"off\" spellcheck=\"false\"><\/td><td><input type=\"text\" name=\"comp7_stock\" aria-label=\"Concentration m\u00e8re, ligne 7\" autocomplete=\"off\" spellcheck=\"false\" inputmode=\"decimal\"><\/td><td><input type=\"text\" name=\"comp7_final\" aria-label=\"Concentration finale, ligne 7\" autocomplete=\"off\" spellcheck=\"false\" inputmode=\"decimal\"><\/td><td><input type=\"text\" name=\"comp7_unit\" aria-label=\"Unit\u00e9 (identique), ligne 7\" autocomplete=\"off\" spellcheck=\"false\"><\/td><\/tr><tr><th scope=\"row\" class=\"a6t-rownum\">8<\/th><td><input type=\"text\" name=\"comp8_name\" aria-label=\"Composant, ligne 8\" autocomplete=\"off\" spellcheck=\"false\"><\/td><td><input type=\"text\" name=\"comp8_stock\" aria-label=\"Concentration m\u00e8re, ligne 8\" autocomplete=\"off\" spellcheck=\"false\" inputmode=\"decimal\"><\/td><td><input type=\"text\" name=\"comp8_final\" aria-label=\"Concentration finale, ligne 8\" autocomplete=\"off\" spellcheck=\"false\" inputmode=\"decimal\"><\/td><td><input type=\"text\" name=\"comp8_unit\" aria-label=\"Unit\u00e9 (identique), ligne 8\" autocomplete=\"off\" spellcheck=\"false\"><\/td><\/tr><\/tbody><\/table><\/div><span class=\"error\" id=\"a6t-melange-pcr-comp-err\" data-a6t-err-for=\"comp1_name comp1_stock comp1_final comp1_unit comp2_name comp2_stock comp2_final comp2_unit comp3_name comp3_stock comp3_final comp3_unit comp4_name comp4_stock comp4_final comp4_unit comp5_name comp5_stock comp5_final comp5_unit comp6_name comp6_stock comp6_final comp6_unit comp7_name comp7_stock comp7_final comp7_unit comp8_name comp8_stock comp8_final comp8_unit\" aria-live=\"polite\"><\/span><\/fieldset>\t\t\t\t<div class=\"a6t-actions\">\n\t\t\t\t\t<button type=\"submit\" class=\"btn btn-primary\">Calculer<\/button>\n\t\t\t\t\t<button type=\"reset\" class=\"btn btn-ghost\">R\u00e9initialiser<\/button>\n\t\t\t\t\t<button type=\"button\" class=\"a6t-linkbtn\" data-a6t-example>Essayer l\u2019exemple<\/button>\n\t\t\t\t<\/div>\n\t\t\t<\/fieldset>\n\t\t\t<p class=\"error a6t-formerror\" data-a6t-err-for=\"_\" role=\"alert\"><\/p>\n\n\t\t\t<div class=\"calc-result a6t-result\" role=\"status\" aria-live=\"polite\" tabindex=\"-1\" hidden>\n\t\t\t\t<div class=\"a6t-main\"><\/div>\n\t\t\t\t<ul class=\"a6t-lines\"><\/ul>\n\t\t\t\t<div class=\"a6t-chart\"><\/div>\n\t\t\t\t<div class=\"a6t-table\"><\/div>\n\t\t\t\t<p class=\"a6t-formula\"><\/p>\n\t\t\t\t<ul class=\"a6t-notes\"><\/ul>\n\t\t\t\t<p class=\"hint\">R\u00e9sultat indicatif, \u00e0 interpr\u00e9ter par un professionnel de sant\u00e9.<\/p>\n\t\t\t\t<div class=\"a6t-result-actions\">\n\t\t\t\t\t<button type=\"button\" class=\"btn btn-ghost\" data-a6t-copy>Copier le r\u00e9sultat<\/button>\n\t\t\t\t\t<span class=\"a6t-copied\" aria-live=\"polite\"><\/span>\n\t\t\t\t<\/div>\n\t\t\t<\/div>\n\n\t\t\t<noscript><p class=\"notice\">Ce calculateur fonctionne dans votre navigateur et n\u00e9cessite JavaScript. La formule et un exemple sont d\u00e9taill\u00e9s ci-dessous.<\/p><\/noscript>\n\t\t\t<script type=\"application\/json\" class=\"a6t-example-data\">{\"vRxn\":\"25\",\"nRxn\":\"10\",\"excess\":\"10\",\"vTemplate\":\"5\",\"comp1_name\":\"Tampon\",\"comp1_stock\":\"10\",\"comp1_final\":\"1\",\"comp1_unit\":\"\\u00d7\",\"comp2_name\":\"MgCl2\",\"comp2_stock\":\"25\",\"comp2_final\":\"1,5\",\"comp2_unit\":\"mM\",\"comp3_name\":\"dNTP\",\"comp3_stock\":\"10\",\"comp3_final\":\"0,2\",\"comp3_unit\":\"mM\",\"comp4_name\":\"Amorce F\",\"comp4_stock\":\"10\",\"comp4_final\":\"0,4\",\"comp4_unit\":\"\\u00b5M\",\"comp5_name\":\"Amorce R\",\"comp5_stock\":\"10\",\"comp5_final\":\"0,4\",\"comp5_unit\":\"\\u00b5M\",\"comp6_name\":\"Polym\\u00e9rase\",\"comp6_stock\":\"5\",\"comp6_final\":\"0,025\",\"comp6_unit\":\"U\\\/\\u00b5L\"}<\/script>\n\t\t<\/form>\n\n\t\t<div class=\"a6t-doc\">\n\t\t\t<h3>Formule<\/h3>\n\t\t\t<div class=\"a6t-formula-box\"><p>Volume par r\u00e9action = concentration finale \/ concentration m\u00e8re \u00d7 volume r\u00e9actionnel<\/p><p>Eau = volume r\u00e9actionnel \u2212 matrice \u2212 somme des composants ; total = volume par r\u00e9action \u00d7 r\u00e9actions \u00d7 (1 + exc\u00e8s).<\/p><\/div>\n\n\t\t\t<h3>Unit\u00e9s<\/h3>\n\t\t\t<p>Volumes en \u00b5L ; pour chaque composant, concentration m\u00e8re et finale dans la m\u00eame unit\u00e9.<\/p>\n\n\t\t\t<h3>Exemple<\/h3>\n\t\t\t<p>Exemple de saisie (concentrations \u00e0 remplacer par celles de votre protocole) : r\u00e9action de 25 \u00b5L dont 5 \u00b5L de matrice, 10 r\u00e9actions + 10 % d\u2019exc\u00e8s : 20 \u00b5L de m\u00e9lange par puits, 220 \u00b5L \u00e0 pr\u00e9parer, dont 13,38 \u00b5L d\u2019eau par r\u00e9action.<\/p>\n\n\t\t\t<h3>Limites<\/h3>\n\t\t\t<ul>\n\t\t\t\t\t\t\t\t\t<li>L\u2019outil ne valide pas la composition chimique du protocole : il calcule les volumes \u00e0 partir des valeurs saisies.<\/li>\n\t\t\t\t\t\t\t\t\t<li>Pr\u00e9parer le m\u00e9lange sur glace, sans la matrice, puis distribuer.<\/li>\n\t\t\t\t\t\t\t<\/ul>\n\n\t\t\t<h3>R\u00e9f\u00e9rences<\/h3>\n\t\t\t<ol class=\"a6t-refs\">\n\t\t\t\t\t\t\t\t\t<li>\n\t\t\t\t\t\t\t\t\t\t\t\t\t<a href=\"https:\/\/doi.org\/10.1373\/clinchem.2008.112797\" rel=\"noopener\" target=\"_blank\">Bustin SA, Benes V, Garson JA, et al. The MIQE guidelines: minimum information for publication of quantitative real-time PCR experiments. Clin Chem. 2009;55(4):611-622.<\/a>\n\t\t\t\t\t\t\t\t\t\t\t<\/li>\n\t\t\t\t\t\t\t\t\t<li>\n\t\t\t\t\t\t\t\t\t\t\t\t\t<a href=\"https:\/\/goldbook.iupac.org\/\" rel=\"noopener\" target=\"_blank\">IUPAC. Compendium of Chemical Terminology (Gold Book) \u2014 concentration en quantit\u00e9 de mati\u00e8re, concentration massique, fraction massique, fraction volumique.<\/a>\n\t\t\t\t\t\t\t\t\t\t\t<\/li>\n\t\t\t\t\t\t\t<\/ol>\n\t\t\t<p class=\"a6t-verified\">Formule v\u00e9rifi\u00e9e par rapport aux r\u00e9f\u00e9rences ci-dessus le 12 octobre 2026.<\/p>\n\t\t<\/div>\n\t<\/section>\n\t<aside class=\"box box-warning a6t-disclaimer\" role=\"note\"><strong>Avertissement :<\/strong> ces outils sont p\u00e9dagogiques. Les calculs sont effectu\u00e9s dans votre navigateur ; aucune valeur n\u2019est enregistr\u00e9e ni transmise. Ils ne posent pas de diagnostic et ne remplacent pas l\u2019avis d\u2019un m\u00e9decin, d\u2019un biologiste ou d\u2019un pharmacien.<\/aside><nav class=\"a6t-related\" aria-labelledby=\"a6t-rel-moleculaire\"><h2 id=\"a6t-rel-moleculaire\">Outils associ\u00e9s<\/h2><ul><li><a href=\"https:\/\/avo6.com\/?page_id=863\">Microbiologie et parasitologie : num\u00e9rations<\/a><\/li><li><a href=\"https:\/\/avo6.com\/?page_id=851\">Calculs de dilution<\/a><\/li><li><a href=\"https:\/\/avo6.com\/?page_id=853\">Pr\u00e9paration de solutions<\/a><\/li><li><a href=\"https:\/\/avo6.com\/?page_id=857\">Tous les outils de biologie m\u00e9dicale<\/a><\/li><\/ul><\/nav><\/div>\n\n","protected":false},"excerpt":{"rendered":"<p>Calculs de paillasse en biologie mol\u00e9culaire. Chaque outil affiche ses hypoth\u00e8ses ; le m\u00e9lange PCR calcule les volumes \u00e0 partir des concentrations de votre\u2026<\/p>\n","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"om_disable_all_campaigns":false,"_monsterinsights_skip_tracking":false,"footnotes":""},"class_list":["post-865","page","type-page","status-publish","hentry"],"aioseo_notices":[],"aioseo_head":"\n\t\t<!-- All in One SEO 5.0.3 - aioseo.com -->\n\t<meta name=\"description\" content=\"Calculs de paillasse en biologie mol\u00e9culaire. 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