{"id":853,"date":"2026-10-12T16:50:35","date_gmt":"2026-10-12T16:50:35","guid":{"rendered":"https:\/\/avo6.com\/?page_id=853"},"modified":"2026-10-12T16:50:35","modified_gmt":"2026-10-12T16:50:35","slug":"preparation-de-solutions","status":"publish","type":"page","link":"https:\/\/avo6.com\/?page_id=853","title":{"rendered":"Pr\u00e9paration de solutions"},"content":{"rendered":"\n<p>Calculs de pr\u00e9paration de r\u00e9actifs : masse \u00e0 peser \u00e0 partir de la masse molaire, concentration obtenue, volume final, solutions exprim\u00e9es en pourcentage, masse molaire d\u2019une formule brute et mise \u00e0 l\u2019\u00e9chelle d\u2019une recette de tampon.<\/p>\n\n<div class=\"a6t-group a6t-has-tabs\" data-a6t-group=\"solutions\"><nav class=\"a6t-tabs\" aria-label=\"Outils de cette page\"><ul><li><a href=\"#molarite\" data-a6t-tab=\"molarite\">Molarit\u00e9<\/a><\/li><li><a href=\"#pourcentages\" data-a6t-tab=\"pourcentages\">Pourcentages m\/V, V\/V, m\/m<\/a><\/li><li><a href=\"#masse-molaire\" data-a6t-tab=\"masse-molaire\">Masse molaire<\/a><\/li><li><a href=\"#recette-solution\" data-a6t-tab=\"recette-solution\">Recette de solution (PBS\u2026)<\/a><\/li><\/ul><\/nav>\t<section class=\"a6t-tool\" id=\"molarite\" data-a6t-panel aria-labelledby=\"a6t-molarite-title\">\n\t\t<h2 id=\"a6t-molarite-title\" class=\"a6t-title\">Molarit\u00e9 : masse \u00e0 peser, concentration ou volume<\/h2>\n\t\t<p class=\"a6t-lead\">m = C \u00d7 V \u00d7 M : masse de r\u00e9actif \u00e0 peser, concentration obtenue ou volume final, avec puret\u00e9 facultative.<\/p>\n\n\t\t<form class=\"calc a6t-form\" data-a6t-tool=\"molarite\" novalidate autocomplete=\"off\" aria-label=\"Calculateur : Molarit\u00e9\">\n\t\t\t\t\t\t<fieldset class=\"a6t-fieldset\" disabled>\n\t\t\t\t<legend class=\"screen-reader-text\">Donn\u00e9es \u00e0 saisir<\/legend>\n\t\t\t\t<fieldset class=\"field a6t-field\"><legend>Je veux calculer<\/legend><div class=\"seg\"><label><input type=\"radio\" name=\"solve\" value=\"m\" checked='checked' aria-describedby=\"a6t-molarite-solve-err\"> <span>la masse<\/span><\/label><label><input type=\"radio\" name=\"solve\" value=\"c\" aria-describedby=\"a6t-molarite-solve-err\"> <span>la concentration<\/span><\/label><label><input type=\"radio\" name=\"solve\" value=\"v\" aria-describedby=\"a6t-molarite-solve-err\"> <span>le volume<\/span><\/label><\/div><span class=\"error\" id=\"a6t-molarite-solve-err\" data-a6t-err-for=\"solve\" aria-live=\"polite\"><\/span><\/fieldset><div class=\"field a6t-field\"><label for=\"a6t-molarite-mw\">Masse molaire M (g\/mol)<\/label><input id=\"a6t-molarite-mw\" name=\"mw\" type=\"text\" inputmode=\"decimal\" autocomplete=\"off\" spellcheck=\"false\" value=\"\" aria-describedby=\"a6t-molarite-mw-hint a6t-molarite-mw-err\" aria-required=\"true\"><span class=\"hint\" id=\"a6t-molarite-mw-hint\">Celle de la forme pes\u00e9e (anhydre ou hydrat\u00e9e).<\/span><span class=\"error\" id=\"a6t-molarite-mw-err\" data-a6t-err-for=\"mw\" aria-live=\"polite\"><\/span><\/div><div class=\"field a6t-field\" data-a6t-computed=\"solve=c\"><label for=\"a6t-molarite-c\">Concentration molaire C<\/label><div class=\"input-unit\"><input id=\"a6t-molarite-c\" name=\"c\" type=\"text\" inputmode=\"decimal\" autocomplete=\"off\" spellcheck=\"false\" value=\"\" aria-describedby=\"a6t-molarite-c-err\" aria-required=\"true\"><select id=\"a6t-molarite-cUnit\" name=\"cUnit\" aria-label=\"Unit\u00e9 \u2014 Concentration molaire C\"><option value=\"mol\/L\" selected='selected'>mol\/L<\/option><option value=\"mmol\/L\">mmol\/L<\/option><option value=\"\u00b5mol\/L\">\u00b5mol\/L<\/option><\/select><\/div><span class=\"error\" id=\"a6t-molarite-c-err\" data-a6t-err-for=\"c cUnit\" aria-live=\"polite\"><\/span><\/div><div class=\"field a6t-field\" data-a6t-computed=\"solve=v\"><label for=\"a6t-molarite-v\">Volume final V<\/label><div class=\"input-unit\"><input id=\"a6t-molarite-v\" name=\"v\" type=\"text\" inputmode=\"decimal\" autocomplete=\"off\" spellcheck=\"false\" value=\"\" aria-describedby=\"a6t-molarite-v-err\" aria-required=\"true\"><select id=\"a6t-molarite-vUnit\" name=\"vUnit\" aria-label=\"Unit\u00e9 \u2014 Volume final V\"><option value=\"L\" selected='selected'>L<\/option><option value=\"mL\">mL<\/option><option value=\"\u00b5L\">\u00b5L<\/option><\/select><\/div><span class=\"error\" id=\"a6t-molarite-v-err\" data-a6t-err-for=\"v vUnit\" aria-live=\"polite\"><\/span><\/div><div class=\"field a6t-field\" data-a6t-computed=\"solve=m\"><label for=\"a6t-molarite-m\">Masse pes\u00e9e m<\/label><div class=\"input-unit\"><input id=\"a6t-molarite-m\" name=\"m\" type=\"text\" inputmode=\"decimal\" autocomplete=\"off\" spellcheck=\"false\" value=\"\" aria-describedby=\"a6t-molarite-m-err\" aria-required=\"true\"><select id=\"a6t-molarite-mUnit\" name=\"mUnit\" aria-label=\"Unit\u00e9 \u2014 Masse pes\u00e9e m\"><option value=\"g\" selected='selected'>g<\/option><option value=\"mg\">mg<\/option><option value=\"\u00b5g\">\u00b5g<\/option><\/select><\/div><span class=\"error\" id=\"a6t-molarite-m-err\" data-a6t-err-for=\"m mUnit\" aria-live=\"polite\"><\/span><\/div><div class=\"field a6t-field\"><label for=\"a6t-molarite-purity\">Puret\u00e9 du r\u00e9actif (%) \u2014 facultatif<\/label><input id=\"a6t-molarite-purity\" name=\"purity\" type=\"text\" inputmode=\"decimal\" autocomplete=\"off\" spellcheck=\"false\" value=\"\" aria-describedby=\"a6t-molarite-purity-hint a6t-molarite-purity-err\"><span class=\"hint\" id=\"a6t-molarite-purity-hint\">100 % si vide.<\/span><span class=\"error\" id=\"a6t-molarite-purity-err\" data-a6t-err-for=\"purity\" aria-live=\"polite\"><\/span><\/div>\t\t\t\t<div class=\"a6t-actions\">\n\t\t\t\t\t<button type=\"submit\" class=\"btn btn-primary\">Calculer<\/button>\n\t\t\t\t\t<button type=\"reset\" class=\"btn btn-ghost\">R\u00e9initialiser<\/button>\n\t\t\t\t\t<button type=\"button\" class=\"a6t-linkbtn\" data-a6t-example>Essayer l\u2019exemple<\/button>\n\t\t\t\t<\/div>\n\t\t\t<\/fieldset>\n\t\t\t<p class=\"error a6t-formerror\" data-a6t-err-for=\"_\" role=\"alert\"><\/p>\n\n\t\t\t<div class=\"calc-result a6t-result\" role=\"status\" aria-live=\"polite\" tabindex=\"-1\" hidden>\n\t\t\t\t<div class=\"a6t-main\"><\/div>\n\t\t\t\t<ul class=\"a6t-lines\"><\/ul>\n\t\t\t\t<div class=\"a6t-chart\"><\/div>\n\t\t\t\t<div class=\"a6t-table\"><\/div>\n\t\t\t\t<p class=\"a6t-formula\"><\/p>\n\t\t\t\t<ul class=\"a6t-notes\"><\/ul>\n\t\t\t\t<p class=\"hint\">R\u00e9sultat indicatif, \u00e0 interpr\u00e9ter par un professionnel de sant\u00e9.<\/p>\n\t\t\t\t<div class=\"a6t-result-actions\">\n\t\t\t\t\t<button type=\"button\" class=\"btn btn-ghost\" data-a6t-copy>Copier le r\u00e9sultat<\/button>\n\t\t\t\t\t<span class=\"a6t-copied\" aria-live=\"polite\"><\/span>\n\t\t\t\t<\/div>\n\t\t\t<\/div>\n\n\t\t\t<noscript><p class=\"notice\">Ce calculateur fonctionne dans votre navigateur et n\u00e9cessite JavaScript. La formule et un exemple sont d\u00e9taill\u00e9s ci-dessous.<\/p><\/noscript>\n\t\t\t<script type=\"application\/json\" class=\"a6t-example-data\">{\"solve\":\"m\",\"mw\":\"58,44\",\"c\":\"0,15\",\"cUnit\":\"mol\\\/L\",\"v\":\"1\",\"vUnit\":\"L\",\"mUnit\":\"g\"}<\/script>\n\t\t<\/form>\n\n\t\t<div class=\"a6t-doc\">\n\t\t\t<h3>Formule<\/h3>\n\t\t\t<div class=\"a6t-formula-box\"><p>m = C \u00d7 V \u00d7 M \u00f7 (puret\u00e9 \/ 100) ; C = m \u00d7 puret\u00e9 \/ (V \u00d7 M) ; V = m \u00d7 puret\u00e9 \/ (C \u00d7 M). Concentration massique (g\/L) = C \u00d7 M.<\/p><\/div>\n\n\t\t\t<h3>Unit\u00e9s<\/h3>\n\t\t\t<p>C en mol\/L, mmol\/L ou \u00b5mol\/L ; V en L, mL ou \u00b5L ; m en g, mg ou \u00b5g ; M en g\/mol. Le r\u00e9sultat est donn\u00e9 dans l\u2019unit\u00e9 choisie pour la grandeur calcul\u00e9e.<\/p>\n\n\t\t\t<h3>Exemple<\/h3>\n\t\t\t<p>Pr\u00e9parer 1 L de NaCl \u00e0 0,15 mol\/L (M = 58,44 g\/mol) : peser 0,15 \u00d7 1 \u00d7 58,44 = 8,766 g, dissoudre puis compl\u00e9ter \u00e0 1 L.<\/p>\n\n\t\t\t<h3>Limites<\/h3>\n\t\t\t<ul>\n\t\t\t\t\t\t\t\t\t<li>V\u00e9rifier la masse molaire sur le flacon (forme hydrat\u00e9e, sel).<\/li>\n\t\t\t\t\t\t\t\t\t<li>Dissoudre puis compl\u00e9ter au volume final dans une fiole jaug\u00e9e.<\/li>\n\t\t\t\t\t\t\t<\/ul>\n\n\t\t\t<h3>R\u00e9f\u00e9rences<\/h3>\n\t\t\t<ol class=\"a6t-refs\">\n\t\t\t\t\t\t\t\t\t<li>\n\t\t\t\t\t\t\t\t\t\t\t\t\t<a href=\"https:\/\/goldbook.iupac.org\/\" rel=\"noopener\" target=\"_blank\">IUPAC. Compendium of Chemical Terminology (Gold Book) \u2014 concentration en quantit\u00e9 de mati\u00e8re, concentration massique, fraction massique, fraction volumique.<\/a>\n\t\t\t\t\t\t\t\t\t\t\t<\/li>\n\t\t\t\t\t\t\t\t\t<li>\n\t\t\t\t\t\t\t\t\t\t\t\t\t<a href=\"https:\/\/www.bipm.org\/fr\/publications\/si-brochure\" rel=\"noopener\" target=\"_blank\">BIPM. Le Syst\u00e8me international d\u2019unit\u00e9s (SI), 9e \u00e9dition.<\/a>\n\t\t\t\t\t\t\t\t\t\t\t<\/li>\n\t\t\t\t\t\t\t<\/ol>\n\t\t\t<p class=\"a6t-verified\">Formule v\u00e9rifi\u00e9e par rapport aux r\u00e9f\u00e9rences ci-dessus le 12 octobre 2026.<\/p>\n\t\t<\/div>\n\t<\/section>\n\t\t<section class=\"a6t-tool\" id=\"pourcentages\" data-a6t-panel aria-labelledby=\"a6t-pourcentages-title\">\n\t\t<h2 id=\"a6t-pourcentages-title\" class=\"a6t-title\">Pourcentages m\/V, V\/V et m\/m<\/h2>\n\t\t<p class=\"a6t-lead\">Quantit\u00e9 de solut\u00e9 pour pr\u00e9parer une solution \u00e0 un pourcentage donn\u00e9, ou pourcentage d\u2019une solution pr\u00e9par\u00e9e.<\/p>\n\n\t\t<form class=\"calc a6t-form\" data-a6t-tool=\"pourcentages\" novalidate autocomplete=\"off\" aria-label=\"Calculateur : Pourcentages m\/V, V\/V, m\/m\">\n\t\t\t\t\t\t<fieldset class=\"a6t-fieldset\" disabled>\n\t\t\t\t<legend class=\"screen-reader-text\">Donn\u00e9es \u00e0 saisir<\/legend>\n\t\t\t\t<div class=\"field a6t-field\"><label for=\"a6t-pourcentages-type\">Type de pourcentage<\/label><select id=\"a6t-pourcentages-type\" name=\"type\" aria-describedby=\"a6t-pourcentages-type-err\"><option value=\"mv\" selected='selected'>m\/V \u2014 g pour 100 mL de solution<\/option><option value=\"vv\">V\/V \u2014 mL pour 100 mL de solution<\/option><option value=\"mm\">m\/m \u2014 g pour 100 g de solution<\/option><\/select><span class=\"error\" id=\"a6t-pourcentages-type-err\" data-a6t-err-for=\"type\" aria-live=\"polite\"><\/span><\/div><fieldset class=\"field a6t-field\"><legend>Je veux<\/legend><div class=\"seg\"><label><input type=\"radio\" name=\"mode\" value=\"preparer\" checked='checked' aria-describedby=\"a6t-pourcentages-mode-err\"> <span>pr\u00e9parer une solution<\/span><\/label><label><input type=\"radio\" name=\"mode\" value=\"calculer\" aria-describedby=\"a6t-pourcentages-mode-err\"> <span>calculer un %<\/span><\/label><\/div><span class=\"error\" id=\"a6t-pourcentages-mode-err\" data-a6t-err-for=\"mode\" aria-live=\"polite\"><\/span><\/fieldset><div class=\"field a6t-field\" data-a6t-when=\"mode=preparer\" hidden><label for=\"a6t-pourcentages-pct\">Pourcentage souhait\u00e9 (%)<\/label><input id=\"a6t-pourcentages-pct\" name=\"pct\" type=\"text\" inputmode=\"decimal\" autocomplete=\"off\" spellcheck=\"false\" value=\"\" aria-describedby=\"a6t-pourcentages-pct-err\" aria-required=\"true\"><span class=\"error\" id=\"a6t-pourcentages-pct-err\" data-a6t-err-for=\"pct\" aria-live=\"polite\"><\/span><\/div><div class=\"field a6t-field\" data-a6t-when=\"mode=calculer;type=mv,mm\" hidden><label for=\"a6t-pourcentages-soluteMass\">Masse de solut\u00e9 (g)<\/label><input id=\"a6t-pourcentages-soluteMass\" name=\"soluteMass\" type=\"text\" inputmode=\"decimal\" autocomplete=\"off\" spellcheck=\"false\" value=\"\" aria-describedby=\"a6t-pourcentages-soluteMass-err\" aria-required=\"true\"><span class=\"error\" id=\"a6t-pourcentages-soluteMass-err\" data-a6t-err-for=\"soluteMass\" aria-live=\"polite\"><\/span><\/div><div class=\"field a6t-field\" data-a6t-when=\"mode=calculer;type=vv\" hidden><label for=\"a6t-pourcentages-soluteVol\">Volume de solut\u00e9 (mL)<\/label><input id=\"a6t-pourcentages-soluteVol\" name=\"soluteVol\" type=\"text\" inputmode=\"decimal\" autocomplete=\"off\" spellcheck=\"false\" value=\"\" aria-describedby=\"a6t-pourcentages-soluteVol-err\" aria-required=\"true\"><span class=\"error\" id=\"a6t-pourcentages-soluteVol-err\" data-a6t-err-for=\"soluteVol\" aria-live=\"polite\"><\/span><\/div><div class=\"field a6t-field\" data-a6t-when=\"type=mv,vv\" hidden><label for=\"a6t-pourcentages-totalVol\">Volume final de solution (mL)<\/label><input id=\"a6t-pourcentages-totalVol\" name=\"totalVol\" type=\"text\" inputmode=\"decimal\" autocomplete=\"off\" spellcheck=\"false\" value=\"\" aria-describedby=\"a6t-pourcentages-totalVol-err\" aria-required=\"true\"><span class=\"error\" id=\"a6t-pourcentages-totalVol-err\" data-a6t-err-for=\"totalVol\" aria-live=\"polite\"><\/span><\/div><div class=\"field a6t-field\" data-a6t-when=\"type=mm\" hidden><label for=\"a6t-pourcentages-totalMass\">Masse totale de solution (g)<\/label><input id=\"a6t-pourcentages-totalMass\" name=\"totalMass\" type=\"text\" inputmode=\"decimal\" autocomplete=\"off\" spellcheck=\"false\" value=\"\" aria-describedby=\"a6t-pourcentages-totalMass-err\" aria-required=\"true\"><span class=\"error\" id=\"a6t-pourcentages-totalMass-err\" data-a6t-err-for=\"totalMass\" aria-live=\"polite\"><\/span><\/div>\t\t\t\t<div class=\"a6t-actions\">\n\t\t\t\t\t<button type=\"submit\" class=\"btn btn-primary\">Calculer<\/button>\n\t\t\t\t\t<button type=\"reset\" class=\"btn btn-ghost\">R\u00e9initialiser<\/button>\n\t\t\t\t\t<button type=\"button\" class=\"a6t-linkbtn\" data-a6t-example>Essayer l\u2019exemple<\/button>\n\t\t\t\t<\/div>\n\t\t\t<\/fieldset>\n\t\t\t<p class=\"error a6t-formerror\" data-a6t-err-for=\"_\" role=\"alert\"><\/p>\n\n\t\t\t<div class=\"calc-result a6t-result\" role=\"status\" aria-live=\"polite\" tabindex=\"-1\" hidden>\n\t\t\t\t<div class=\"a6t-main\"><\/div>\n\t\t\t\t<ul class=\"a6t-lines\"><\/ul>\n\t\t\t\t<div class=\"a6t-chart\"><\/div>\n\t\t\t\t<div class=\"a6t-table\"><\/div>\n\t\t\t\t<p class=\"a6t-formula\"><\/p>\n\t\t\t\t<ul class=\"a6t-notes\"><\/ul>\n\t\t\t\t<p class=\"hint\">R\u00e9sultat indicatif, \u00e0 interpr\u00e9ter par un professionnel de sant\u00e9.<\/p>\n\t\t\t\t<div class=\"a6t-result-actions\">\n\t\t\t\t\t<button type=\"button\" class=\"btn btn-ghost\" data-a6t-copy>Copier le r\u00e9sultat<\/button>\n\t\t\t\t\t<span class=\"a6t-copied\" aria-live=\"polite\"><\/span>\n\t\t\t\t<\/div>\n\t\t\t<\/div>\n\n\t\t\t<noscript><p class=\"notice\">Ce calculateur fonctionne dans votre navigateur et n\u00e9cessite JavaScript. La formule et un exemple sont d\u00e9taill\u00e9s ci-dessous.<\/p><\/noscript>\n\t\t\t<script type=\"application\/json\" class=\"a6t-example-data\">{\"type\":\"mv\",\"mode\":\"preparer\",\"pct\":\"0,9\",\"totalVol\":\"500\"}<\/script>\n\t\t<\/form>\n\n\t\t<div class=\"a6t-doc\">\n\t\t\t<h3>Formule<\/h3>\n\t\t\t<div class=\"a6t-formula-box\"><p>% m\/V = g de solut\u00e9 \/ 100 mL de solution ; % V\/V = mL de solut\u00e9 \/ 100 mL de solution ; % m\/m = g de solut\u00e9 \/ 100 g de solution.<\/p><p>Solut\u00e9 = % \u00d7 quantit\u00e9 totale \/ 100.<\/p><\/div>\n\n\t\t\t<h3>Unit\u00e9s<\/h3>\n\t\t\t<p>g et mL ; le pourcentage se rapporte toujours \u00e0 la solution finale, pas au solvant.<\/p>\n\n\t\t\t<h3>Exemple<\/h3>\n\t\t\t<p>500 mL de NaCl \u00e0 0,9 % m\/V : 0,9 \u00d7 500 \/ 100 = 4,5 g de NaCl, compl\u00e9ter \u00e0 500 mL.<\/p>\n\n\t\t\t<h3>Limites<\/h3>\n\t\t\t<ul>\n\t\t\t\t\t\t\t\t\t<li>Les volumes ne sont pas toujours additifs (\u00e9thanol + eau) : compl\u00e9ter au trait de jauge.<\/li>\n\t\t\t\t\t\t\t\t\t<li>Ne pas confondre % m\/V et % m\/m pour les solutions denses.<\/li>\n\t\t\t\t\t\t\t<\/ul>\n\n\t\t\t<h3>R\u00e9f\u00e9rences<\/h3>\n\t\t\t<ol class=\"a6t-refs\">\n\t\t\t\t\t\t\t\t\t<li>\n\t\t\t\t\t\t\t\t\t\t\t\t\t<a href=\"https:\/\/goldbook.iupac.org\/\" rel=\"noopener\" target=\"_blank\">IUPAC. Compendium of Chemical Terminology (Gold Book) \u2014 concentration en quantit\u00e9 de mati\u00e8re, concentration massique, fraction massique, fraction volumique.<\/a>\n\t\t\t\t\t\t\t\t\t\t\t<\/li>\n\t\t\t\t\t\t\t<\/ol>\n\t\t\t<p class=\"a6t-verified\">Formule v\u00e9rifi\u00e9e par rapport aux r\u00e9f\u00e9rences ci-dessus le 12 octobre 2026.<\/p>\n\t\t<\/div>\n\t<\/section>\n\t\t<section class=\"a6t-tool\" id=\"masse-molaire\" data-a6t-panel aria-labelledby=\"a6t-masse-molaire-title\">\n\t\t<h2 id=\"a6t-masse-molaire-title\" class=\"a6t-title\">Masse molaire \u00e0 partir d\u2019une formule brute<\/h2>\n\t\t<p class=\"a6t-lead\">Masse molaire et composition massique d\u2019une formule brute, hydrates et parenth\u00e8ses compris (ex. CaCl\u2082\u00b72H\u2082O).<\/p>\n\n\t\t<form class=\"calc a6t-form\" data-a6t-tool=\"masse-molaire\" novalidate autocomplete=\"off\" aria-label=\"Calculateur : Masse molaire\">\n\t\t\t\t\t\t<fieldset class=\"a6t-fieldset\" disabled>\n\t\t\t\t<legend class=\"screen-reader-text\">Donn\u00e9es \u00e0 saisir<\/legend>\n\t\t\t\t<div class=\"field a6t-field\"><label for=\"a6t-masse-molaire-formula\">Formule brute<\/label><input id=\"a6t-masse-molaire-formula\" name=\"formula\" type=\"text\" autocomplete=\"off\" spellcheck=\"false\" value=\"\" aria-describedby=\"a6t-masse-molaire-formula-hint a6t-masse-molaire-formula-err\" aria-required=\"true\" placeholder=\"ex. CaCl2\u00b72H2O\"><span class=\"hint\" id=\"a6t-masse-molaire-formula-hint\">Majuscule en d\u00e9but de symbole ; \u00ab \u00b7 \u00bb ou \u00ab . \u00bb pour l\u2019eau d\u2019hydratation.<\/span><span class=\"error\" id=\"a6t-masse-molaire-formula-err\" data-a6t-err-for=\"formula\" aria-live=\"polite\"><\/span><\/div>\t\t\t\t<div class=\"a6t-actions\">\n\t\t\t\t\t<button type=\"submit\" class=\"btn btn-primary\">Calculer<\/button>\n\t\t\t\t\t<button type=\"reset\" class=\"btn btn-ghost\">R\u00e9initialiser<\/button>\n\t\t\t\t\t<button type=\"button\" class=\"a6t-linkbtn\" data-a6t-example>Essayer l\u2019exemple<\/button>\n\t\t\t\t<\/div>\n\t\t\t<\/fieldset>\n\t\t\t<p class=\"error a6t-formerror\" data-a6t-err-for=\"_\" role=\"alert\"><\/p>\n\n\t\t\t<div class=\"calc-result a6t-result\" role=\"status\" aria-live=\"polite\" tabindex=\"-1\" hidden>\n\t\t\t\t<div class=\"a6t-main\"><\/div>\n\t\t\t\t<ul class=\"a6t-lines\"><\/ul>\n\t\t\t\t<div class=\"a6t-chart\"><\/div>\n\t\t\t\t<div class=\"a6t-table\"><\/div>\n\t\t\t\t<p class=\"a6t-formula\"><\/p>\n\t\t\t\t<ul class=\"a6t-notes\"><\/ul>\n\t\t\t\t<p class=\"hint\">R\u00e9sultat indicatif, \u00e0 interpr\u00e9ter par un professionnel de sant\u00e9.<\/p>\n\t\t\t\t<div class=\"a6t-result-actions\">\n\t\t\t\t\t<button type=\"button\" class=\"btn btn-ghost\" data-a6t-copy>Copier le r\u00e9sultat<\/button>\n\t\t\t\t\t<span class=\"a6t-copied\" aria-live=\"polite\"><\/span>\n\t\t\t\t<\/div>\n\t\t\t<\/div>\n\n\t\t\t<noscript><p class=\"notice\">Ce calculateur fonctionne dans votre navigateur et n\u00e9cessite JavaScript. La formule et un exemple sont d\u00e9taill\u00e9s ci-dessous.<\/p><\/noscript>\n\t\t\t<script type=\"application\/json\" class=\"a6t-example-data\">{\"formula\":\"CaCl2\\u00b72H2O\"}<\/script>\n\t\t<\/form>\n\n\t\t<div class=\"a6t-doc\">\n\t\t\t<h3>Formule<\/h3>\n\t\t\t<div class=\"a6t-formula-box\"><p>M = \u03a3 (nombre d\u2019atomes \u00d7 masse atomique standard)<\/p><\/div>\n\n\t\t\t<h3>Unit\u00e9s<\/h3>\n\t\t\t<p>g\/mol. Poids atomiques abr\u00e9g\u00e9s CIAAW 2024.<\/p>\n\n\t\t\t<h3>Exemple<\/h3>\n\t\t\t<p>CaCl\u2082\u00b72H\u2082O : 40,078 + 2 \u00d7 35,45 + 2 \u00d7 18,015 = 147,01 g\/mol.<\/p>\n\n\t\t\t<h3>Limites<\/h3>\n\t\t\t<ul>\n\t\t\t\t\t\t\t\t\t<li>Les \u00e9l\u00e9ments sans masse atomique standard (Tc, Pm, Po\u2026) ne sont pas accept\u00e9s.<\/li>\n\t\t\t\t\t\t\t\t\t<li>Pour un r\u00e9actif commercial, la masse molaire indiqu\u00e9e par le fabricant (forme exacte, hydratation) pr\u00e9vaut.<\/li>\n\t\t\t\t\t\t\t<\/ul>\n\n\t\t\t<h3>R\u00e9f\u00e9rences<\/h3>\n\t\t\t<ol class=\"a6t-refs\">\n\t\t\t\t\t\t\t\t\t<li>\n\t\t\t\t\t\t\t\t\t\t\t\t\t<a href=\"https:\/\/ciaaw.org\/abridged-atomic-weights.htm\" rel=\"noopener\" target=\"_blank\">CIAAW (IUPAC). Abridged Standard Atomic Weights 2024.<\/a>\n\t\t\t\t\t\t\t\t\t\t\t<\/li>\n\t\t\t\t\t\t\t<\/ol>\n\t\t\t<p class=\"a6t-verified\">Formule v\u00e9rifi\u00e9e par rapport aux r\u00e9f\u00e9rences ci-dessus le 12 octobre 2026.<\/p>\n\t\t<\/div>\n\t<\/section>\n\t\t<section class=\"a6t-tool\" id=\"recette-solution\" data-a6t-panel aria-labelledby=\"a6t-recette-solution-title\">\n\t\t<h2 id=\"a6t-recette-solution-title\" class=\"a6t-title\">Pr\u00e9paration d\u2019un tampon ou d\u2019une solution selon une recette<\/h2>\n\t\t<p class=\"a6t-lead\">Quantit\u00e9s pour un volume et une concentration (1\u00d7, 10\u00d7\u2026) donn\u00e9s, \u00e0 partir d\u2019une recette document\u00e9e (PBS) ou de votre recette.<\/p>\n\n\t\t<form class=\"calc a6t-form\" data-a6t-tool=\"recette-solution\" novalidate autocomplete=\"off\" aria-label=\"Calculateur : Recette de solution (PBS\u2026)\">\n\t\t\t\t\t\t<fieldset class=\"a6t-fieldset\" disabled>\n\t\t\t\t<legend class=\"screen-reader-text\">Donn\u00e9es \u00e0 saisir<\/legend>\n\t\t\t\t<div class=\"field a6t-field\"><label for=\"a6t-recette-solution-preset\">Recette<\/label><select id=\"a6t-recette-solution-preset\" name=\"preset\" aria-describedby=\"a6t-recette-solution-preset-err\"><option value=\"pbs\" selected='selected'>PBS \u2014 Cold Spring Harbor Protocols (pour 1 L \u00e0 1\u00d7)<\/option><option value=\"custom\">Ma recette<\/option><\/select><span class=\"error\" id=\"a6t-recette-solution-preset-err\" data-a6t-err-for=\"preset\" aria-live=\"polite\"><\/span><\/div><div class=\"field a6t-field\" data-a6t-when=\"preset=custom\" hidden><label for=\"a6t-recette-solution-vRef\">Volume de ma recette<\/label><div class=\"input-unit\"><input id=\"a6t-recette-solution-vRef\" name=\"vRef\" type=\"text\" inputmode=\"decimal\" autocomplete=\"off\" spellcheck=\"false\" value=\"\" aria-describedby=\"a6t-recette-solution-vRef-err\" aria-required=\"true\"><select id=\"a6t-recette-solution-refUnit\" name=\"refUnit\" aria-label=\"Unit\u00e9 \u2014 Volume de ma recette\"><option value=\"L\" selected='selected'>L<\/option><option value=\"mL\">mL<\/option><\/select><\/div><span class=\"error\" id=\"a6t-recette-solution-vRef-err\" data-a6t-err-for=\"vRef refUnit\" aria-live=\"polite\"><\/span><\/div><fieldset class=\"field a6t-field a6t-rows\" data-a6t-when=\"preset=custom\" hidden><legend>Ingr\u00e9dients de ma recette<\/legend><div class=\"a6t-rows-scroll\"><table><thead><tr><th scope=\"col\"><span class=\"screen-reader-text\">N\u00b0<\/span><\/th><th scope=\"col\">Ingr\u00e9dient<\/th><th scope=\"col\">Quantit\u00e9<\/th><th scope=\"col\">Unit\u00e9<\/th><\/tr><\/thead><tbody><tr><th scope=\"row\" class=\"a6t-rownum\">1<\/th><td><input type=\"text\" name=\"ing1_name\" aria-label=\"Ingr\u00e9dient, ligne 1\" autocomplete=\"off\" spellcheck=\"false\"><\/td><td><input type=\"text\" name=\"ing1_qty\" aria-label=\"Quantit\u00e9, ligne 1\" autocomplete=\"off\" spellcheck=\"false\" inputmode=\"decimal\"><\/td><td><select name=\"ing1_unit\" aria-label=\"Unit\u00e9, ligne 1\"><option value=\"g\">g<\/option><option value=\"mg\">mg<\/option><option value=\"\u00b5g\">\u00b5g<\/option><option value=\"mL\">mL<\/option><option value=\"\u00b5L\">\u00b5L<\/option><\/select><\/td><\/tr><tr><th scope=\"row\" class=\"a6t-rownum\">2<\/th><td><input type=\"text\" name=\"ing2_name\" aria-label=\"Ingr\u00e9dient, ligne 2\" autocomplete=\"off\" spellcheck=\"false\"><\/td><td><input type=\"text\" name=\"ing2_qty\" aria-label=\"Quantit\u00e9, ligne 2\" autocomplete=\"off\" spellcheck=\"false\" inputmode=\"decimal\"><\/td><td><select name=\"ing2_unit\" aria-label=\"Unit\u00e9, ligne 2\"><option value=\"g\">g<\/option><option value=\"mg\">mg<\/option><option value=\"\u00b5g\">\u00b5g<\/option><option value=\"mL\">mL<\/option><option value=\"\u00b5L\">\u00b5L<\/option><\/select><\/td><\/tr><tr><th scope=\"row\" class=\"a6t-rownum\">3<\/th><td><input type=\"text\" name=\"ing3_name\" aria-label=\"Ingr\u00e9dient, ligne 3\" autocomplete=\"off\" spellcheck=\"false\"><\/td><td><input type=\"text\" name=\"ing3_qty\" aria-label=\"Quantit\u00e9, ligne 3\" autocomplete=\"off\" spellcheck=\"false\" inputmode=\"decimal\"><\/td><td><select name=\"ing3_unit\" aria-label=\"Unit\u00e9, ligne 3\"><option value=\"g\">g<\/option><option value=\"mg\">mg<\/option><option value=\"\u00b5g\">\u00b5g<\/option><option value=\"mL\">mL<\/option><option value=\"\u00b5L\">\u00b5L<\/option><\/select><\/td><\/tr><tr><th scope=\"row\" class=\"a6t-rownum\">4<\/th><td><input type=\"text\" name=\"ing4_name\" aria-label=\"Ingr\u00e9dient, ligne 4\" autocomplete=\"off\" spellcheck=\"false\"><\/td><td><input type=\"text\" name=\"ing4_qty\" aria-label=\"Quantit\u00e9, ligne 4\" autocomplete=\"off\" spellcheck=\"false\" inputmode=\"decimal\"><\/td><td><select name=\"ing4_unit\" aria-label=\"Unit\u00e9, ligne 4\"><option value=\"g\">g<\/option><option value=\"mg\">mg<\/option><option value=\"\u00b5g\">\u00b5g<\/option><option value=\"mL\">mL<\/option><option value=\"\u00b5L\">\u00b5L<\/option><\/select><\/td><\/tr><tr><th scope=\"row\" class=\"a6t-rownum\">5<\/th><td><input type=\"text\" name=\"ing5_name\" aria-label=\"Ingr\u00e9dient, ligne 5\" autocomplete=\"off\" spellcheck=\"false\"><\/td><td><input type=\"text\" name=\"ing5_qty\" aria-label=\"Quantit\u00e9, ligne 5\" autocomplete=\"off\" spellcheck=\"false\" inputmode=\"decimal\"><\/td><td><select name=\"ing5_unit\" aria-label=\"Unit\u00e9, ligne 5\"><option value=\"g\">g<\/option><option value=\"mg\">mg<\/option><option value=\"\u00b5g\">\u00b5g<\/option><option value=\"mL\">mL<\/option><option value=\"\u00b5L\">\u00b5L<\/option><\/select><\/td><\/tr><tr><th scope=\"row\" class=\"a6t-rownum\">6<\/th><td><input type=\"text\" name=\"ing6_name\" aria-label=\"Ingr\u00e9dient, ligne 6\" autocomplete=\"off\" spellcheck=\"false\"><\/td><td><input type=\"text\" name=\"ing6_qty\" aria-label=\"Quantit\u00e9, ligne 6\" autocomplete=\"off\" spellcheck=\"false\" inputmode=\"decimal\"><\/td><td><select name=\"ing6_unit\" aria-label=\"Unit\u00e9, ligne 6\"><option value=\"g\">g<\/option><option value=\"mg\">mg<\/option><option value=\"\u00b5g\">\u00b5g<\/option><option value=\"mL\">mL<\/option><option value=\"\u00b5L\">\u00b5L<\/option><\/select><\/td><\/tr><tr><th scope=\"row\" class=\"a6t-rownum\">7<\/th><td><input type=\"text\" name=\"ing7_name\" aria-label=\"Ingr\u00e9dient, ligne 7\" autocomplete=\"off\" spellcheck=\"false\"><\/td><td><input type=\"text\" name=\"ing7_qty\" aria-label=\"Quantit\u00e9, ligne 7\" autocomplete=\"off\" spellcheck=\"false\" inputmode=\"decimal\"><\/td><td><select name=\"ing7_unit\" aria-label=\"Unit\u00e9, ligne 7\"><option value=\"g\">g<\/option><option value=\"mg\">mg<\/option><option value=\"\u00b5g\">\u00b5g<\/option><option value=\"mL\">mL<\/option><option value=\"\u00b5L\">\u00b5L<\/option><\/select><\/td><\/tr><tr><th scope=\"row\" class=\"a6t-rownum\">8<\/th><td><input type=\"text\" name=\"ing8_name\" aria-label=\"Ingr\u00e9dient, ligne 8\" autocomplete=\"off\" spellcheck=\"false\"><\/td><td><input type=\"text\" name=\"ing8_qty\" aria-label=\"Quantit\u00e9, ligne 8\" autocomplete=\"off\" spellcheck=\"false\" inputmode=\"decimal\"><\/td><td><select name=\"ing8_unit\" aria-label=\"Unit\u00e9, ligne 8\"><option value=\"g\">g<\/option><option value=\"mg\">mg<\/option><option value=\"\u00b5g\">\u00b5g<\/option><option value=\"mL\">mL<\/option><option value=\"\u00b5L\">\u00b5L<\/option><\/select><\/td><\/tr><\/tbody><\/table><\/div><span class=\"error\" id=\"a6t-recette-solution-ing-err\" data-a6t-err-for=\"ing1_name ing1_qty ing1_unit ing2_name ing2_qty ing2_unit ing3_name ing3_qty ing3_unit ing4_name ing4_qty ing4_unit ing5_name ing5_qty ing5_unit ing6_name ing6_qty ing6_unit ing7_name ing7_qty ing7_unit ing8_name ing8_qty ing8_unit\" aria-live=\"polite\"><\/span><\/fieldset><div class=\"field a6t-field\"><label for=\"a6t-recette-solution-vTarget\">Volume \u00e0 pr\u00e9parer<\/label><div class=\"input-unit\"><input id=\"a6t-recette-solution-vTarget\" name=\"vTarget\" type=\"text\" inputmode=\"decimal\" autocomplete=\"off\" spellcheck=\"false\" value=\"\" aria-describedby=\"a6t-recette-solution-vTarget-err\" aria-required=\"true\"><select id=\"a6t-recette-solution-vUnit\" name=\"vUnit\" aria-label=\"Unit\u00e9 \u2014 Volume \u00e0 pr\u00e9parer\"><option value=\"mL\" selected='selected'>mL<\/option><option value=\"L\">L<\/option><\/select><\/div><span class=\"error\" id=\"a6t-recette-solution-vTarget-err\" data-a6t-err-for=\"vTarget vUnit\" aria-live=\"polite\"><\/span><\/div><div class=\"field a6t-field\"><label for=\"a6t-recette-solution-factor\">Concentration voulue (\u00d7 la recette) \u2014 facultatif<\/label><input id=\"a6t-recette-solution-factor\" name=\"factor\" type=\"text\" inputmode=\"decimal\" autocomplete=\"off\" spellcheck=\"false\" value=\"\" aria-describedby=\"a6t-recette-solution-factor-hint a6t-recette-solution-factor-err\"><span class=\"hint\" id=\"a6t-recette-solution-factor-hint\">1 si vide ; 10 pour une solution m\u00e8re 10\u00d7.<\/span><span class=\"error\" id=\"a6t-recette-solution-factor-err\" data-a6t-err-for=\"factor\" aria-live=\"polite\"><\/span><\/div>\t\t\t\t<div class=\"a6t-actions\">\n\t\t\t\t\t<button type=\"submit\" class=\"btn btn-primary\">Calculer<\/button>\n\t\t\t\t\t<button type=\"reset\" class=\"btn btn-ghost\">R\u00e9initialiser<\/button>\n\t\t\t\t\t<button type=\"button\" class=\"a6t-linkbtn\" data-a6t-example>Essayer l\u2019exemple<\/button>\n\t\t\t\t<\/div>\n\t\t\t<\/fieldset>\n\t\t\t<p class=\"error a6t-formerror\" data-a6t-err-for=\"_\" role=\"alert\"><\/p>\n\n\t\t\t<div class=\"calc-result a6t-result\" role=\"status\" aria-live=\"polite\" tabindex=\"-1\" hidden>\n\t\t\t\t<div class=\"a6t-main\"><\/div>\n\t\t\t\t<ul class=\"a6t-lines\"><\/ul>\n\t\t\t\t<div class=\"a6t-chart\"><\/div>\n\t\t\t\t<div class=\"a6t-table\"><\/div>\n\t\t\t\t<p class=\"a6t-formula\"><\/p>\n\t\t\t\t<ul class=\"a6t-notes\"><\/ul>\n\t\t\t\t<p class=\"hint\">R\u00e9sultat indicatif, \u00e0 interpr\u00e9ter par un professionnel de sant\u00e9.<\/p>\n\t\t\t\t<div class=\"a6t-result-actions\">\n\t\t\t\t\t<button type=\"button\" class=\"btn btn-ghost\" data-a6t-copy>Copier le r\u00e9sultat<\/button>\n\t\t\t\t\t<span class=\"a6t-copied\" aria-live=\"polite\"><\/span>\n\t\t\t\t<\/div>\n\t\t\t<\/div>\n\n\t\t\t<noscript><p class=\"notice\">Ce calculateur fonctionne dans votre navigateur et n\u00e9cessite JavaScript. La formule et un exemple sont d\u00e9taill\u00e9s ci-dessous.<\/p><\/noscript>\n\t\t\t<script type=\"application\/json\" class=\"a6t-example-data\">{\"preset\":\"pbs\",\"vTarget\":\"1\",\"vUnit\":\"L\"}<\/script>\n\t\t<\/form>\n\n\t\t<div class=\"a6t-doc\">\n\t\t\t<h3>Formule<\/h3>\n\t\t\t<div class=\"a6t-formula-box\"><p>Quantit\u00e9 = quantit\u00e9 de la recette \u00d7 (volume \u00e0 pr\u00e9parer \/ volume de la recette) \u00d7 facteur de concentration.<\/p><p>PBS 1\u00d7 (pour 1 L) : NaCl 8 g ; KCl 0,2 g ; Na\u2082HPO\u2084 1,44 g ; KH\u2082PO\u2084 0,24 g ; pH 7,4.<\/p><\/div>\n\n\t\t\t<h3>Unit\u00e9s<\/h3>\n\t\t\t<p>Volumes en mL ou L ; quantit\u00e9s en g, mg, \u00b5g, mL ou \u00b5L.<\/p>\n\n\t\t\t<h3>Exemple<\/h3>\n\t\t\t<p>1 L de PBS 1\u00d7 : 8 g de NaCl, 0,2 g de KCl, 1,44 g de Na\u2082HPO\u2084 et 0,24 g de KH\u2082PO\u2084, pH ajust\u00e9 \u00e0 7,4.<\/p>\n\n\t\t\t<h3>Limites<\/h3>\n\t\t\t<ul>\n\t\t\t\t\t\t\t\t\t<li>Les masses de la recette PBS correspondent aux sels anhydres ; pour une forme hydrat\u00e9e, recalculez avec l\u2019outil Molarit\u00e9.<\/li>\n\t\t\t\t\t\t\t\t\t<li>Une solution concentr\u00e9e (10\u00d7) peut pr\u00e9cipiter au froid : la r\u00e9chauffer avant usage.<\/li>\n\t\t\t\t\t\t\t<\/ul>\n\n\t\t\t<h3>R\u00e9f\u00e9rences<\/h3>\n\t\t\t<ol class=\"a6t-refs\">\n\t\t\t\t\t\t\t\t\t<li>\n\t\t\t\t\t\t\t\t\t\t\t\t\t<a href=\"https:\/\/doi.org\/10.1101\/pdb.rec8247\" rel=\"noopener\" target=\"_blank\">Phosphate-buffered saline (PBS). Cold Spring Harbor Protocols. 2006. doi:10.1101\/pdb.rec8247.<\/a>\n\t\t\t\t\t\t\t\t\t\t\t<\/li>\n\t\t\t\t\t\t\t<\/ol>\n\t\t\t<p class=\"a6t-verified\">Formule v\u00e9rifi\u00e9e par rapport aux r\u00e9f\u00e9rences ci-dessus le 12 octobre 2026.<\/p>\n\t\t<\/div>\n\t<\/section>\n\t<aside class=\"box box-warning a6t-disclaimer\" role=\"note\"><strong>Avertissement :<\/strong> ces outils sont p\u00e9dagogiques. Les calculs sont effectu\u00e9s dans votre navigateur ; aucune valeur n\u2019est enregistr\u00e9e ni transmise. Ils ne posent pas de diagnostic et ne remplacent pas l\u2019avis d\u2019un m\u00e9decin, d\u2019un biologiste ou d\u2019un pharmacien.<\/aside><nav class=\"a6t-related\" aria-labelledby=\"a6t-rel-solutions\"><h2 id=\"a6t-rel-solutions\">Outils associ\u00e9s<\/h2><ul><li><a href=\"https:\/\/avo6.com\/?page_id=851\">Calculs de dilution<\/a><\/li><li><a href=\"https:\/\/avo6.com\/?page_id=849\">Convertisseur d\u2019unit\u00e9s biologiques<\/a><\/li><li><a href=\"https:\/\/avo6.com\/?page_id=857\">Tous les outils de biologie m\u00e9dicale<\/a><\/li><\/ul><\/nav><\/div>\n\n","protected":false},"excerpt":{"rendered":"<p>Calculs de pr\u00e9paration de r\u00e9actifs : masse \u00e0 peser \u00e0 partir de la masse molaire, concentration obtenue, volume final, solutions exprim\u00e9es en pourcentage, masse\u2026<\/p>\n","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"om_disable_all_campaigns":false,"_monsterinsights_skip_tracking":false,"footnotes":""},"class_list":["post-853","page","type-page","status-publish","hentry"],"aioseo_notices":[],"aioseo_head":"\n\t\t<!-- All in One SEO 5.0.3 - aioseo.com -->\n\t<meta name=\"description\" content=\"Calculs de pr\u00e9paration de r\u00e9actifs : masse \u00e0 peser \u00e0 partir de la masse molaire, concentration obtenue, volume final, solutions exprim\u00e9es en pourcentage, masse molaire d\u2019une formule brute et mise \u00e0 l\u2019\u00e9chelle d\u2019une recette de tampon.\" \/>\n\t<meta name=\"robots\" content=\"max-image-preview:large\" \/>\n\t<link rel=\"canonical\" href=\"https:\/\/avo6.com\/?page_id=853\" \/>\n\t\t<meta name=\"generator\" content=\"All in One SEO (AIOSEO) 5.0.3\" \/>\n\t\t<meta property=\"og:locale\" content=\"fr_FR\" \/>\n\t\t<meta property=\"og:site_name\" content=\"Le Monde Des Analyses M\u00e9dicales \u2013 Si vous souhaitez en savoir plus sur les analyses m\u00e9dicales et leur fonctionnement ? 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